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Key Documents
PRON-RO
Roche
Pronase
from Streptomyces griseus
Synonim(y):
non-specific protease
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About This Item
Polecane produkty
pochodzenie biologiczne
Streptomyces griseus
Poziom jakości
sterylność
non-sterile
Postać
lyophilized powder
opakowanie
pkg of 1 g (10165921001)
pkg of 5 g (11459643001)
producent / nazwa handlowa
Roche
stężenie
0.5-2 mg/mL
Parametry
35-40 °C optimum reaction temp.
metody
DNA extraction: suitable
kolor
white
optymalne pH
7.0-8.0
zakres pH
6.0-9.0
rozpuszczalność
water: soluble 10-20 g/L
przydatność
suitable for molecular biology
numer dostępu NCBI
Zastosowanie
life science and biopharma
obecność zanieczyszczeń
Deoxyribonuclease <0.002 U/mg ( based on lyoph.)
Ribonuclease <0.002 U/mg ( based on lyoph.)
temp. przechowywania
2-8°C
informacje o genach
Streptomyces griseus ... SGR_RS27460(6214424)
Opis ogólny
at 40°C with casein as the substrate, pH 7.5, equivalent to approximately 1270 PU/mg or approximately 25 PUK/mg.
Specyficzność
Pronase has a broad specificity, breaking down virtually all proteins into their individual amino acids; resolves carboxylic acids and alcohols.
Zastosowanie
Use Pronase to completely hydrolyze proteins in research applications. Pronase is used for the degradation of proteins during the isolation of DNA and RNA, such as in the extraction of phage DNA or the isolation of plasmid DNA. It is not necessary to let pronase self-digest prior to use. It is also used in histochemistry and cell culture for tissue dissociation in conjunction with collagenase and trypsin, and for the production of glycopeptides from purified glycoproteins. It has been used for the dechorionation of Zebrafish embryos.
Działania biochem./fizjol.
Pronase has wide-ranging specificity. Therefore, it is commonly used when extensive or complete degradation of proteins is required. Its applications include protein analysis in cell organelles, removal of proteins during DNA and RNA isolation, and the production of protein hydrolysates for amino acid analysis. In addition, Pronase surpasses trypsin in its ability to disperse fibroblastic cell lines, excelling in both monolayer detachment and the creation of single-cell suspensions.
Definicja jednostki
A unit of non-specific protease activity increases the rate of release of folin-positive amino acids and peptides from casein, at +40 °C and pH 7.5. For one unit (U) 1 μmol/min tyrosine is released, and for the unit "PU", the value is 1 μg/minute (1 U = 181 PU); for the unit "PUK", it is 0.1/minute (measured is the change in absorbance of molybdenum blue, formed by reaction with the folin ′s reagent under conditions such that 1 PUK = 50 PU).
Uwaga dotycząca przygotowania
Stabilizers: Protective effect of calcium ions
The preparation contains 20% calcium acetate for stability. It is free of starch as per the current Quality Control Procedures.The activity of a diluted solution containing 0.01–0.1 M calcium was stable over 24 hours at neutral pH at 4 to 8 °C. Pronase is also protected from heat inactivation by low levels of calcium.
Working concentration: 0.5 to 2 mg/ml
Working solution: Solvent is recommended in distilled water.
Stock solution is prepared by adding pronase powder to distilled water (10 to 20 mg/ml).
Storage conditions (working solution): -15 to -25 °C
The preparation contains 20% calcium acetate for stability. It is free of starch as per the current Quality Control Procedures.The activity of a diluted solution containing 0.01–0.1 M calcium was stable over 24 hours at neutral pH at 4 to 8 °C. Pronase is also protected from heat inactivation by low levels of calcium.
Working concentration: 0.5 to 2 mg/ml
Working solution: Solvent is recommended in distilled water.
Stock solution is prepared by adding pronase powder to distilled water (10 to 20 mg/ml).
Storage conditions (working solution): -15 to -25 °C
Rekonstytucja
The lyophilizate is soluble in water (10 to 20 mg/ml).
Przechowywanie i stabilność
Store dry
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
This page may contain text that has been machine translated.
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organy docelowe
Respiratory system
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
does not flash
Temperatura zapłonu (°C)
does not flash
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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