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Merck

K4002

Sigma-Aldrich

Krebs-Ringer Bicarbonate Buffer

With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

Synonim(y):

Bicarbonate Buffer Solution, Krebs Buffer, Krebs-Ringer Solution

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About This Item

Kod UNSPSC:
12161702
NACRES:
NA.75

Poziom jakości

Formularz

powder

metody

cell culture | mammalian: suitable

pH

6.1-6.7

rozpuszczalność

water: 9.5 g/L, clear

temp. przechowywania

2-8°C

Opis ogólny

Krebs-Ringer Bicarbonate Buffer acts as a salt solution and plays an essential role in tissue culture. A salt solution provides the cells with water and inorganic ions. It also helps in maintaining the osmotic balance and ideal pH in the medium. This bicarbonate buffered isotonic saline contains magnesium, potassium, sodium, and phosphate ions.

Zastosowanie

Krebs-Ringer Bicarbonate Buffer has been used:

  • as a wash buffer for the preparation of mice primary adipocytes and lipolysis assay
  • in the pre-perifusion for islet viability and functionality studies
  • in pituitary bioassay

Ilość

Formulated to contain 9.5 grams of powder per liter of medium.

Rekonstytucja

Supplement with 1.26 g/L sodium bicarbonate.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Odwiedź Bibliotekę dokumentów

Bidisha Dasgupta et al.
European journal of immunology, 38(12), 3499-3507 (2008-11-11)
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PloS one, 7(8), e38117-e38117 (2012-08-18)
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Scientific reports, 11(1), 20700-20700 (2021-10-21)
Ethanol provides a rapid, low-cost ablative solution for liver tumors with a small technological footprint but suffers from uncontrolled diffusion in target tissue, limiting treatment precision and accuracy. Incorporating the gel-forming polymer ethyl cellulose to ethanol localizes the distribution. The
Heng Xu et al.
Synapse (New York, N.Y.), 61(3), 166-175 (2006-12-08)
Previous studies established that Tyr-D-Ala-Gly-N-Me-Phe-Gly-ol (DAMGO) and (2S,4aR,6aR,7R,9S,10aS,10bR)-9-(Benzoyloxy)-2-(3-furanyl)dodecahydro-6a,10b-dimethyl-4,10-dioxo-2H-naphtho-[2,1-c]pyran-7-carboxylic acid methyl ester (herkinorin) are fully efficacious mu-agonists. Herkinorin (HERK), unlike DAMGO, does not recruit beta-arrestin and promote mu-receptor internalization, even in cells that over express beta-arrestin. We hypothesized that chronic HERK

Protokoły

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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