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Kluczowe dokumenty
C5670
Calcium chloride
anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%
Synonim(y):
CaCl2, Dichlorek wapnia
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About This Item
Wzór liniowy:
CaCl2
Numer CAS:
Masa cząsteczkowa:
110.98
Numer WE:
Numer MDL:
Kod UNSPSC:
12352207
Identyfikator substancji w PubChem:
NACRES:
NA.71
Polecane produkty
klasa czystości
anhydrous
Poziom jakości
ciśnienie pary
0.01 mmHg ( 20 °C)
linia produktu
BioReagent
Próba
≥96.0%
Formularz
powder
metody
cell culture | insect: suitable
cell culture | plant: suitable
mp
772 °C (lit.)
ciąg SMILES
[Cl-].[Cl-].[Ca++]
InChI
1S/Ca.2ClH/h;2*1H/q+2;;/p-2
Klucz InChI
UXVMQQNJUSDDNG-UHFFFAOYSA-L
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Opis ogólny
Calcium chloride is a commonly used reagent in biochemistry. Calcium plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability.1,2 Extensive reviews of the experimental measurement of biological calcium have been published.3,4Calcium chloride is used in the preparation and transformation of competent E. coli and in the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA.5 The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied.6 A protocol for the concentration of virus vectors that uses CaCl2 has been published.7CaCl2 has been used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from Escherichia coli.8 The crystallization of porcine pancreatic elastase in the presence of CaCl2 in the presence of sodium citrate reveals binding of calcium in the metal binding site of the protein.9 The effect of CaCl2 on the total fluorescence in the polymerication of the tubulin-like FtsZ division protein of Escherichia coli has been studied by a multiwell fluorescent assay.10
Zastosowanie
This product is insect cell culture tested (0.5 mg/ml) and is appropriate for insect cell culture applications. Calcium chloride is used in the preparation and transformation of competent E. coli and in the
transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. A protocol for the concentration of virus vectors that uses CaCl2 has been published.
transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. A protocol for the concentration of virus vectors that uses CaCl2 has been published.
Uwaga dotycząca przygotowania
This product is soluble in water (222 mg/ml, 2 M), yielding a clear, colorless solution.
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Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Irrit. 2
Kod klasy składowania
13 - Non Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Produkty
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Wapń jest jonowo stabilnym dwuwartościowym kationem o ważnych korzystnych i toksycznych właściwościach w hodowli komórkowej. Jest składnikiem szerokiej gamy pożywek do hodowli komórkowych.
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
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