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GE17-0921-07

IMAC Sepharose 6 Fast Flow

Cytiva 17-0921-07, pack of 25 mL

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About This Item

Kod UNSPSC:
41106500
NACRES:
NA.56

opakowanie

pack of 25 mL

producent / nazwa handlowa

Cytiva 17-0921-07

macierz

6% cross-linked agarose

wielkość cząstki

45-165 μm

średnia średnica

90 μm

cleaning in place

2-14(Medium uncharged with metal ions.)

working range

3-12

pojemność

~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

przydatność

suitable for bioprocess medium

Opis ogólny

IMAC Sepharose 6 Fast FLow is a BioProcess IMAC medium, designed for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also untagged recombinant or native proteins.

Zastosowanie

IMAC Sepharose 6 Fast FLow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.


IMAC Sepharose 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with a novel chelating ligand immobilized to the base matrix. IMAC Sepharose 6 Fast FLow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target Protein.


The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.


IMAC Sepharose 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, IMAC Sepharose 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support

Cechy i korzyści

  • Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity
  • Convenient purification of histidine-tagged Proteins, also when Ni2+ is not the best choice of metal ion
  • As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • Available in the convenient and time-saving prepacked HiTrap® and HiPrep formats.

Przechowywanie i stabilność

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Komentarz do analizy

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informacje prawne

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
HiPrep is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
This page may contain text that has been machine translated.

Piktogramy

Flame

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Kod klasy składowania

3 - Flammable liquids


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Produkty

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from GE Healthcare.

This page shows a general procedure for cell lysis of recombinant histidine-tagged proteins using GE Healthcare products.

Postępuj zgodnie z ustalonymi procedurami ekspresji białek rekombinowanych, wzrostu, indukcji i optymalizacji lizy komórek.

This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.

Zobacz wszystko

Protokoły

This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from GE Healthcare.

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using GE Healthcare products.

Zobacz wszystko

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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