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G7017
β-Glucuronidase from Helix pomatia
Type HP-2, aqueous solution, ≥100,000 units/mL
Synonim(y):
β-D-Glucuronide glucuronosohydrolase
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About This Item
Numer CAS:
Numer MDL:
Kod UNSPSC:
12352204
NACRES:
NA.54
Polecane produkty
pochodzenie biologiczne
Helix pomatia
Poziom jakości
typ
Type HP-2
Formularz
aqueous solution
aktywność właściwa
≥100,000 units/mL
secondary activity
≤7,500 units/mL sulfatase
Warunki transportu
wet ice
temp. przechowywania
2-8°C
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Opis ogólny
β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the digestive juices of the Roman snail. β-glucuronidases derived from mollusks often contain sulfatase activity. Both activities are useful in the hydrolysis of metabolites for toxicology and biomarker analysis. For a broader look at our β-Glucuronidase product line and their uses see our page about Drug Metabolite Analysis
Zastosowanie
- Sample preparation for LC/MS through digestion of glucuronide conjugated compounds for biomarker and toxicology research.
- Removal of glucuronide conjugates such as for the preparation of Baicalein.
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Działania biochem./fizjol.
Endogenous beta-glucuronidase is a lysosomal enzyme involved in digestion of glucuronide-modified catabolites.
Microbial beta-glucuronidase found in microbiota can cause re-absorption of metabolites by hydrolysis of glucuronide conjugates of xenobiotic and endobiotic metabolites.
Microbial beta-glucuronidase found in microbiota can cause re-absorption of metabolites by hydrolysis of glucuronide conjugates of xenobiotic and endobiotic metabolites.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Cechy i korzyści
- pH optima 4.0 - 5.0.
- Sulfatase pH optima 6.2
- Low Sulfatase activity (<7,500 units/mL).
- Ammonium Sulfate suspension.
- Mollusk-derived beta-glucuronidase.
- Useful for acidic hydrolysis of glucuronides in low pH samples.
Jakość
Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Powiązanie
Similar to G0876, but produced by Sigma.
Definicja jednostki
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
Postać fizyczna
Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.
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Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Resp. Sens. 1
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
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Produkty
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
Protokoły
Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver
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