G7017

β-Glucuronidase from Helix pomatia

Type HP-2, aqueous solution, ≥100,000 units/mL

• Synonim(y): β-D-Glucuronide glucuronosohydrolase
• Numer CAS: 9001-45-0
• Numer EC enzymu: 3.2.1.31 (BRENDA, IUBMB)
• Numer MDL: MFCD00130629
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• pochodzenie biologiczne: Helix pomatia
• typ: Type HP-2
• Postać: aqueous solution
• aktywność właściwa: ≥100,000 units/mL
• secondary activity: ≤7,500 units/mL sulfatase
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the digestive juices of the Roman snail. β-glucuronidases derived from mollusks often contain sulfatase activity. Both activities are useful in the hydrolysis of metabolites for toxicology and biomarker analysis. For a broader look at our β-Glucuronidase product line and their uses see our page about Drug Metabolite Analysis

Zastosowanie

• Sample preparation for LC/MS through digestion of glucuronide conjugated compounds for biomarker and toxicology research.
• Removal of glucuronide conjugates such as for the preparation of Baicalein.­

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Działania biochem./fizjol.

Endogenous beta-glucuronidase is a lysosomal enzyme involved in digestion of glucuronide-modified catabolites.
Microbial beta-glucuronidase found in microbiota can cause re-absorption of metabolites by hydrolysis of glucuronide conjugates of xenobiotic and endobiotic metabolites.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Cechy i korzyści

• pH optima 4.0 - 5.0.
• Sulfatase pH optima 6.2
• Low Sulfatase activity (<7,500 units/mL).
• Ammonium Sulfate suspension.
• Mollusk-derived beta-glucuronidase.
• Useful for acidic hydrolysis of glucuronides in low pH samples.

Jakość

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Powiązanie

Similar to G0876, but produced by Sigma.

Definicja jednostki

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Postać fizyczna

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H317,H334
• Zwroty wskazujące środki ostrożności: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Klasyfikacja zagrożeń: Resp. Sens. 1 - Skin Sens. 1
• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3