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G8885

Sigma-Aldrich

β-Glucuronidase from Helix pomatia

Type H-3, aqueous solution, ≥90,000 units/mL

Synonim(y):

β-D-Glucuronide glucuronosohydrolase

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About This Item

Numer CAS:
9001-45-0
Numer EC enzymu:
3.2.1.31 (BRENDA, IUBMB)
Numer MDL:
MFCD00130629
Kod UNSPSC:
12352204
NACRES:
NA.54

typ

Type H-3

Postać

aqueous solution

aktywność właściwa

≥90,000 units/mL

secondary activity

≤1,000 units/mL sulfatase

Warunki transportu

wet ice

temp. przechowywania

2-8°C

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Opis ogólny

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Zastosowanie

β-Glucuronidase from Helix pomatia has been used:
  • to convert isoflavone conjugates to their aglycone forms in urine and soymilk samples for high performance liquid chromatography (HPLC) detection
  • as a component in the protoplasting solution
  • for the generation of aromatic and phenolic compounds from the plasma and urine samples for gas chromatography-mass spectrometry (GC-MS)
  • to hydrolyze glucuronide and sulfate conjugated metabolites in fecal samples

β-glucuronidase was used in the measurement of aromatization by a urine technique suitable for the evaluation of aromatase inhibitors in vivo.
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Działania biochem./fizjol.

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Jakość

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Definicja jednostki

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Postać fizyczna

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.
This page may contain text that has been machine translated.

Piktogramy

Health hazard
GHS08

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H317,H334

Zwroty wskazujące środki ostrożności

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Klasyfikacja zagrożeń

Resp. Sens. 1 - Skin Sens. 1

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

A metabolite profiling approach to identify biomarkers of flavonoid intake in humans
Loke WM, et al.
The Journal of Nutrition, 139(12), 2309-2314 (2009)
The zrfA and zrfB genes of Aspergillus fumigatus encode the zinc transporter proteins of a zinc uptake system induced in an acid, zinc-depleted environment
Vicentefranqueira R, et al.
Eukaryotic Cell, 4(5), 837-848 (2005)
S Jacobs et al.
Journal of enzyme inhibition, 4(4), 315-325 (1991-01-01)
By modification of a recently developed method for separation of radio-labelled urinary oestrogens we were able to separate oestrogen metabolites and measure their isotope ratios in urine following injections of [3H]delta 4-androstenedione and [14C]oestrone. This method provides a useful tool
Equol producer status, salivary estradiol profile and urinary excretion of isoflavones in Irish Caucasian women, following ingestion of soymilk
Hall MC, et al.
Steroids, 72(1), 64-70 (2007)
Andrew M Jenner et al.
Free radical biology & medicine, 38(6), 763-772 (2005-02-22)
Phenolic compounds are not completely absorbed in the small intestine and so enter the colon, where they might exert physiological effects. To identify phenolics that are present in normal human colon, fecal water was prepared from 5 free-living volunteers with
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Produkty

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Protokoły

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver

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