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G6795
Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal
clone GSN24, purified from hybridoma cell culture
Synonim(y):
Anty-GFP
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About This Item
Kod UNSPSC:
12352203
NACRES:
NA.56
Polecane produkty
pochodzenie biologiczne
mouse
białko sprzężone
unconjugated
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
GSN24, monoclonal
Formularz
buffered aqueous solution
masa cząsteczkowa
antigen 27 kDa
stężenie
~2 mg/mL
metody
western blot: 1-2 μg/mL using GFP fusion proteins expressed in extracts of transfected cells
izotyp
IgG1
Warunki transportu
dry ice
temp. przechowywania
−20°C
2-8°C
docelowa modyfikacja potranslacyjna
unmodified
Opis ogólny
Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma GSN24 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice.
Specyficzność
The antibody reacts specifically with GFP fusion proteins.
Zastosowanie
Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal has been used in: western blotting, enzyme linked immunosorbent assay (ELISA), immunofluorescence labeling.
Działania biochem./fizjol.
Green Fluorescent Protein (GFP) is a unique tool in cellular and molecular biology research to monitor gene expression and protein localization in living cells. In the jellyfish, A. victoria, GFP transduces the excitation energy resulting from emission of blue light of the photoprotein aequorin and reemits it as green light.
Postać fizyczna
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Przechowywanie i stabilność
For continuous use, store at 2–8 °C for up to one month. For extended storage, store at –20 °C in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Numer produktu
Opis
Cennik
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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