Wszystkie zdjęcia(2)
Kluczowe dokumenty
E9408
p3xFLAG-Myc-CMV™-25 Expression Vector
Shuttle vector for transient or stable expression of secreted dual tagged N-terminal 3xFLAG and C-terminal c-myc fusion proteins
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352200
Polecane produkty
znacznik
3X FLAG tagged
c-Myc tagged (C-terminal)
klasa czystości
for molecular biology
Formularz
buffered aqueous solution
Warunki transportu
dry ice
temp. przechowywania
−20°C
Opis ogólny
The p3XFLAG-Myc-CMV™-25 Expression Vector is a 6.4 kb derivative of pCMV5 used to establish transient or stable expression of secreted, dual tagged N-terminal 3XFLAG™and C-terminal c-myc fusion proteins in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) and a c-myc epitope (EQKLISEEDL) upstream and downstream of the multiple cloning sites, respectively. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein. The incorporation of 3XFLAG in the expression vector results in increased detection sensitivity using ANTI-FLAG M2 antibody.
p3XFLAG-Myc-CMV-25 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication is optimal when using an SV40 T antigen expressing host.
The p3XFLAG-CMV-7-BAP Control Plasmid is a 6.2 kb derivative of pCMV5 used for transient intracellular expression of N-terminal 3XFLAG bacterial alkaline phosphatase fusion protein in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein.
Vector Maps and Sequences
p3XFLAG-Myc-CMV-25 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication is optimal when using an SV40 T antigen expressing host.
The p3XFLAG-CMV-7-BAP Control Plasmid is a 6.2 kb derivative of pCMV5 used for transient intracellular expression of N-terminal 3XFLAG bacterial alkaline phosphatase fusion protein in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein.
Vector Maps and Sequences
Komponenty
p3XFLAG-Myc-CMV™-25 Expression Vector 20 μg (E6276) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
p3XFLAG-CMV™-7-BAP Control Plasmid 20 μg (C7472) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
p3XFLAG-CMV™-7-BAP Control Plasmid 20 μg (C7472) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
Zasada
The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG® and c-myc fusion constructs. The aminoglycoside phosphotransferase II gene (Neo-r) confers resistance to aminoglycosides such as G418 allowing for selection of stable transfectants.
Informacje prawne
This product is covered by the following patents owned by Sigma-Aldrich Co. LLC: US6,379,903, US7,094,548, JP4405125,EP1220933, CA2386471 and AU774216.
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
p3xFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
p3xFLAG-Myc-CMV is a trademark of Sigma-Aldrich Co. LLC
Ta strona może zawierać tekst przetłumaczony maszynowo.
Kod klasy składowania
10 - Combustible liquids
Wybierz jedną z najnowszych wersji:
Certyfikaty analizy (CoA)
Lot/Batch Number
Przepraszamy, ale COA dla tego produktu nie jest aktualnie dostępny online.
Proszę o kontakt, jeśli potrzebna jest pomoc Obsługa Klienta
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Wenqin Xu et al.
Journal of virology, 85(7), 3498-3506 (2011-01-29)
BHK cells remain resistant to xenotropic murine retrovirus-related virus (XMRV) or gibbon ape leukemia virus (GALV) infection, even when their respective receptors, Xpr1 or PiT1, are expressed. We set out to determine the stage at which viral infection is blocked
P A Possik et al.
European journal of histochemistry : EJH, 48(3), 267-272 (2004-12-14)
We used immunocytochemical and fluorescence assays to investigate the subcellular location of the protein encoded by Down syndrome critical region gene 2 (DSCR2) in transfected cells. It was previously suggested that DSCR2 is located in the plasma membrane as an
Mitsuyoshi Hashimoto et al.
Glycobiology, 17(9), 994-1006 (2007-06-27)
Secreted mucins protect the underlying epithelium by serving as the major determinant of the rheological property of mucus secretion and the receptors for pathogens. These functions can be affected by the three branch structures, including core 2, core 4, and
Li-Yun Jia et al.
Investigative ophthalmology & visual science, 50(8), 3743-3749 (2009-02-24)
To characterize a novel Asp384Asn (D384N) mutant myocilin (MYOC) that causes juvenile-onset open-angle glaucoma (JOAG) and investigate the correction of mutant phenotype by a natural osmolyte, trimethylamine N-oxide (TMAO). A Chinese JOAG family was recruited and genomic DNA was extracted
Masahiro Nakagawa et al.
Blood, 108(10), 3329-3334 (2006-08-05)
The Notch1-RBP-Jkappa and the transcription factor Runx1 pathways have been independently shown to be indispensable for the establishment of definitive hematopoiesis. Importantly, expression of Runx1 is down-regulated in the para-aortic splanchnopleural (P-Sp) region of Notch1- and Rbpsuh-null mice. Here we
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej