D8168

Anti-Dystrophin Antibody

mouse monoclonal, MANDYS8

• Synonim(y): Przeciwciało dystrofiny, Przeciwciało przeciwko dystrofinie - monoklonalne przeciwciało przeciwko dystrofinie wytwarzane u myszy
• Numer MDL: MFCD00164586
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• Nazwa produktu: Monoclonal Anti-Dystrophin antibody produced in mouse, clone MANDYS8, ascites fluid
• pochodzenie biologiczne: mouse
• Poziom jakości: 200
• białko sprzężone: unconjugated
• forma przeciwciała: ascites fluid
• rodzaj przeciwciała: primary antibodies
• klon: MANDYS8, monoclonal
• reaktywność gatunkowa: chicken, rat, human, pig, rabbit, mouse
• metody: indirect ELISA: suitable; indirect immunofluorescence: 1:400 using frozen human or animal muscle tissue sections.; microarray: suitable; western blot: suitable
• izotyp: IgG2b
• numer dostępu UniProt: P11532
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... DMD(1756) ; mouse ... Dmd(13405) ; rat ... Dmd(24907)

Opis

Opis ogólny

Monoclonal Anti-Dystrophin (mouse IgG2b isotype) is derived from the MANDYS8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Dystrophin is a structural protein on the inner face of the membrane, consisting of a 25-repeat, rod-like, triple-helical domain separating an N-terminal actin binding domain from two C-terminal domains, one of which is rich in cysteine.

Immunogen

recombinant human dystrophin fragment.

Zastosowanie

Monoclonal Anti-Dystrophin antibody produced in mouse has been used in

• immunohistochemistry
• immunofluorescence
• double immunofluorescence terminal dUTP nick-end labeling (TUNEL)
• immunoblotting

Monoclonal anti-dystrophin antibody can be used for localization of dystrophin using immunochemical assays like ELISA and immunohistochemistry. The antibody can also be used in immunoblotting for brain dystrophin. Further, it can be used in western blotting and double immunofluorescent labelling (diluted 1: 500) of dystrophin.

Działania biochem./fizjol.

Dystrophin deficiency is associated with severe Duchenne muscular dystrophy (DMD). Becker muscular dystrophy (BMD) show less pronounced abnormalities of dystrophin protein expression. Since abnormalities in the protein expression occur specifically in patients with these types of muscular dystrophy, dystrophin analysis may be used to distinguish these conditions from other neuromuscular diseases.

Opis wartości docelowych

The rod domain of the human dystrophin molecule is present in normal muscle tissue and in nearly all Becker muscular dystrophies. It is absent in the cases of Duchenne muscular dystrophies and in the dystrophic mouse (mdx).

Inne uwagi

This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200764 Anti-Dystrophin antibody, Mouse monoclonal
clone MANDYS8, purified from hybridoma cell culture

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable