CLLS1007

SW48 Cells TP53 -/-

Human female colon, colorectal adenocarcinoma

• Kod UNSPSC: 41106514
• NACRES: NA.81

Właściwości

• Nazwa produktu: SW48 Cells TP53 -/-,
• pochodzenie biologiczne: human female colon (Source Disease: Colorectal adenocarcinoma)
• Poziom jakości: 200
• numer dostępu OMIM: 191170
• temp. przechowywania: −196°C
• informacje o genach: human ... TP53(7157)

Opis

Opis ogólny

SW48 Cells TP53 -/- are colorectal adenocarcinoma, epithelial cells, from a human caucasian female (aged 82 years), with a ZFN knock out modification.

Zastosowanie

Media Renewal changes one to two times per week

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:2 to 1:6

The base medium for this cell line is DMEM, Cat. No. D5671. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%, Sodium pyruvate, Cat. No. S8636, at a final concentration of 1 mM, and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.

The cell freezing medium is DMSO 1X (Cat. No. C6164)

Cechy i korzyści

These SW48 cells are adherent, with a doubling time of approx. 35 hours.

Zinc finger nuclease (ZFN) knock out on chromosome 17p13.1

Jakość

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

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Elementy zestawu są też dostępne oddzielnie

• SW48 Cells TP53 -/- 1 vial
• SW48 Control Cells 1 vial