JVM-2

96090515, human unknown/unspecified, Lymphoblastoid

• Kod UNSPSC: 41106514

Właściwości

• product name: JVM-2, 96090515
• pochodzenie biologiczne: human unknown/unspecified
• tryb wzrostu: Suspension
• kariotyp: 46,XX,-8,+t(3;8)(p12-13;q12),t(11;14)(q13;q32)
• morfologia: Lymphoblastoid
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Caucasian B-prolymphocytic leukaemia, EBV-transformed

Opis linii komórkowej

JVM-2 has been derived from EBV and TPA treated mononuclear cells from a 63 year old female patient with B-prolymphocytic leukaemia. Thirty-three percent of cells were shown to be positive for surface lambda light chain, 37% for IgA, 29% for IgG and 3% for IgM and IgD. In addition, 50% were found to express cytoplasmic IgM and lambda light chain. Expression of the following leukocyte differentiation antigens was found: CD9, CD19, CD20, CD23, CD24, MA6, CD37, CD38, FMC1, FMC7, MHC Class I, FMC16, MHC Class II DR/DP/DQ. The immunoglobulin heavy-and light-chain genes showed the same pattern of rearrangement as in the original prolymphocytes. The cells are lymphoblastoid with large floating aggregates present. The cell line has been eradicated from mycoplasma at ECACC.

Zastosowanie

Ig expression of neoplastic cells

Profil DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,13
D16S539: 12,13
D5S818: 11,12
D7S820: 10,11
THO1: 6,9
TPOX: 8,11
vWA: 17

pożywka hodowlana

RPMI 1640 +2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Maintain cultures between 3-9 x 100,000/ml; 5% CO₂; 37°C.

Inne uwagi

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