LNCap Clone FGC Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89110211, human prostate (carcinoma), Epithelial-like

• Synonim(y): Komórki LNCaP-FGC
• Kod UNSPSC: 41106514

Właściwości

• pochodzenie biologiczne: human prostate (carcinoma)
• opakowanie: tube of 5 μg 89110211-DNA-5UG; pkg of vial of cells 89110211-1VL
• tryb wzrostu: Adherent
• kariotyp: Pseudodiploid male; seven marker chromosomes, modal number 46, range 33 to 91
• morfologia: Epithelial-like
• produkty: Prostate acid phosphatase, prostate specific antigen
• receptory: Androgen, estrogen
• metody: cell culture | mammalian: suitable
• powiązane choroby: metastasis
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Caucasian prostate carcinoma

Opis linii komórkowej

Derived from a metastasis at the left supraclavicular lymph node of a 50 year old patient with a confirmed diagnosis of metastatic prostate carcinoma. Growth and acid phosphatase production is affected by 5-alpha-dihydrotestosterone. They do not form a uniform monolayer and attach only lightly to the substrate. When shipped, cells detach from flask and can either be incubated 24-48 hours to allow attachment or be collected by centrifugation (150xg, 15 minutes) and reseeded.

Zastosowanie

LNCap Clone FGC Cell Line has been used:

• to study the effect of sex steroid hormones in regulating the expression of oligoadenylate synthetase 1 (OAS1)
• to study the role of curcumin and resveratrol in redox control and ability to promote apoptosis
• to investigate low molecular weight thiols present in the deproteinized extract of the cell line after derivatization

pożywka hodowlana

RPMI 1640 + 2mM Glutamine + 1.0 mM sodium pyruvate + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.i.e. seeding at 1-3 x 10,000 cells/cm² Cells grow slowly in clusters and can be disaggregated by repeated pipetting. After subculture cells may take up to 48 hours to reattach. Do not disturb during this period. Medium is rapidly acidified, therefore change twice per week.

Inne uwagi

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Ta strona może zawierać tekst przetłumaczony maszynowo.