FGC4

94101904, rat skin, Epithelial

• Kod UNSPSC: 41106514

Właściwości

• product name: FGC4, 94101904
• pochodzenie biologiczne: rat skin
• tryb wzrostu: Adherent
• kariotyp: Modal no. 52
• morfologia: Epithelial
• produkty: Albumin and ß-fibrogen
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Rat hepatoma [alb-gpt plasmid]

Opis linii komórkowej

A subclone derived from Fao which is deficient in HGPRT (resistant to 5μg/ml 6-thioguanine and also resistant to 3mM ouabain). FGC4 contains the alb-gpt plasmid which is the bacterial gpt gene under control of the rat albumin promoter (400 bp). Produces albumin and ß-fibrinogen.GMO1

Zastosowanie

Gene expression studies.

pożywka hodowlana

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split semi-confluent cultures (70%) 1:100 i.e. seeding at 2x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Media change three times a week.

Inne uwagi

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