AT-3.1

94101451, rat skin, Epithelial

• Kod UNSPSC: 41106514

Właściwości

• product name: AT-3.1, 94101451
• pochodzenie biologiczne: rat skin
• tryb wzrostu: Adherent
• kariotyp: Hyperaneuploidy
• morfologia: Epithelial
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Rat dorsal prostatic adenocarcinoma

Opis linii komórkowej

AT-3.1 is a subclone of the AT-3 cell line being one of a series of related variants originating from a single spontaneous adenocarcinoma in the dorsal lobe of the prostate of an aged male inbred Copenhagen rat. AT-3.1 was derived from an anaplastic tumour with a high rate of metastases and is androgen-independent.

Zastosowanie

Carcinogenesis studies, cytogenetic analysis.

pożywka hodowlana

RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 2-4 x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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