SK-N-DZ

94092305, human nerve (bone marrow metastasis), Neuronal

• Kod UNSPSC: 41106514

Właściwości

• product name: SK-N-DZ, 94092305
• pochodzenie biologiczne: human nerve (bone marrow metastasis)
• tryb wzrostu: Adherent
• kariotyp: 44XX, monosomic for 10, 11, 13, 14, 19. Both copies of chromosome 2 missing in each cell.
• morfologia: Neuronal
• produkty: p53, moderate MDR-1 expression
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: metastasis
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human neuroblastoma

Opis linii komórkowej

Spontaneously transformed cells from a female patient with neuroblastoma. The tumour was derived from a bone marrow metastasis and is tumourigenic in nude mice. Used in the study of MIBG uptake in the diagnosis and treatment of neuroblastoma. MIBG is a derivative of the antihypertensive agent guanethidine and is used as a radiopharmaceutical. SK-N-DZ is characterised by low saturable kinetics and predominant linear pattern of uptake of MIBG, with a poor ability to maintain high intracellular levels of the drug.

Zastosowanie

Tumour studies. MRD-1 resistance studies. Extremely sensitive to cis-retinoic acid.

Profil DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 8,11
D16S539: 9,11
D5S818: 12
D7S820: 12,13
THO1: 6,9
TPOX: 8
vWA: 16,18

pożywka hodowlana

DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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