TGP 52

94022429, mouse pancreas, Trabecular

• Kod UNSPSC: 41106514

Właściwości

• Nazwa produktu: TGP 52, 94022429
• pochodzenie biologiczne: mouse pancreas
• tryb wzrostu: Adherent
• kariotyp: Not specified
• morfologia: Trabecular
• produkty: Somatostatin
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Mouse islet cell tumour

Opis linii komórkowej

TGP 52 has been isolated from an islet tumour arising in the pancreas of a transgenic Tg (E1a-1-SV40E) Bri18 mouse. These mice carry a pseudogene construct composed of a pancreatic specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 52 cells are SV40T antigen positive and secrete somatostatin, it is one of a series of pancreatic tumour lines deposited at ECACC. Cells transiently produce insulin in culture followed by somatostatin.

Zastosowanie

Study of endocrine pancreas carcinomas, hormone assays.

pożywka hodowlana

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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