CFPAC-1

91112501, human pancreas (liver metastasis), Epithelial

• Kod UNSPSC: 41106514

Właściwości

• product name: CFPAC-1, 91112501
• pochodzenie biologiczne: human pancreas (liver metastasis)
• tryb wzrostu: Adherent
• kariotyp: Hyperdiploid, (65-75 chromosomes)
• morfologia: Epithelial
• produkty: Not specified
• receptory: Cystic fibrosis trans-membrane regulator (CFTR)
• metody: cell culture | mammalian: suitable
• powiązane choroby: metastasis
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Caucasian pancreatic adenocarcinoma

Opis linii komórkowej

CFPAC-1 is a ductal pancreatic adenocarcinoma derived by differential trypsinisation of explant cultures from a metastatic lesion in the liver of a 26 year old caucasian male with cystic fibrosis (CF). The cell line expresses cystic fibrosis trans-membrane regulator (CFTR) and ion transport properties consistent with the CF defect. CFPAC-1 cells show an epithelial morphology and polarisation with apical microvilli. Expression of cytokeratin and oncofoetal antigens characteristic of pancreatic duct cells have been described. The cells express the CF gene and show the most common CF mutation, deletion of three nucleotides result in a phenylalanine-508 deletion. CFPAC-1 cells are also tumorigenic in nude mice.

Zastosowanie

Cystic fibrosis studies, genetic analysis

pożywka hodowlana

IDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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