RO82-W-1

92030502, human thyroid, Epithelial

• Kod UNSPSC: 41106514

Właściwości

• Nazwa produktu: RO82-W-1, 92030502
• pochodzenie biologiczne: human thyroid
• tryb wzrostu: Adherent
• kariotyp: 2n = 60-82, 82 predominant
• morfologia: Epithelial
• produkty: Thyroglobulin
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: metastasis
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human follicular thryoid carcinoma

Opis linii komórkowej

Established from the metastases of a follicular carcinoma in a female patient. Tumorigenic in nude mice. The basal release of immuno-detectable thyroglobulin increased in response to thyroid-stimulating hormone (TSH). The cell line grows as a monolayer without the formation of follicles. Electronmicroscopy studies showed the presence of microvilli with interdigitations between neighbouring cells.

Zastosowanie

A valuable model for the study of human follicular carcinoma.

pożywka hodowlana

DMEM:Ham′s F12:MCDB 105 (2:1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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