GS-109-V-63

90110503, human skin, Fibroblast

• Kod UNSPSC: 41106514

Właściwości

• product name: GS-109-V-63, 90110503
• pochodzenie biologiczne: human skin
• tryb wzrostu: Adherent
• kariotyp: Modal no. 46
• morfologia: Fibroblast
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Caucasian skin fibroblast Gardner′s syndrome

Opis linii komórkowej

Derived from a 13 year old Caucasian male with Gardner′s syndrome, an autosomal dominant condition which predisposes to carcinoma and multiple polyps of the colon. The modal chromosome number was 46, occurring in 60% of cells.

Zastosowanie

Tumourigenicity studies

Profil DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11
D13S317: 11,12
D16S539: 12,13
D5S818: 11,12
D7S820: 9,10
THO1: 7,8
TPOX: 8,11
vWA: 14,17

pożywka hodowlana

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split semi-confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Monolayer should form in a 25cm² flask in 3-4 days.

Inne uwagi

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