AV3 (HeLa derivative)

88102402, human cervix, Epithelial

• Kod UNSPSC: 41106514

Właściwości

• product name: AV3 (HeLa derivative), 88102402
• pochodzenie biologiczne: human cervix
• tryb wzrostu: Adherent
• kariotyp: 2n = 46
• morfologia: Epithelial
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human cervix carcinoma

Opis linii komórkowej

This cell line was originally derived from normal human amnion. The cells are mainly used for virus studies (susceptible to poliovirus 1). HeLa marker chromosomes and type A G6PD have been detected. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line must be considered to be derived from HeLa.

Zastosowanie

Virus studies: poliovirus 1

Profil DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 12
THO1: 7
TPOX: 8,12
vWA: 16,18

pożywka hodowlana

EMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x100,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Inne uwagi

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