DAUDI Cell Line human

85011437, human blood, Lymphoblast

• Synonim(y): DAUDI Cell Culture, Human DAUDI Cells
• Kod UNSPSC: 41106514

Właściwości

• Nazwa produktu: DAUDI Cell Line human, 85011437, African-American Burkitt′s lymphoma, from human blood
• pochodzenie biologiczne: human blood
• tryb wzrostu: Suspension
• kariotyp: 2n = 46, diploid
• morfologia: Lymphoblast
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Burkitt′s lymphoma

Opis linii komórkowej

Derived from a 16 year old male. Positive for EBNA, carries EBV markers, complement receptors, surface bound immunoglobulin and surface markers for the Fc fragment of IgG. The cells should be handled under laboratory containment level 2. Ethnicity: Black.

Zastosowanie

Tumourigenicity and Virus studies: EBV used to study leukemogenesis mechanisms.

Profil DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11,12
D16S539: 10,12
D5S818: 8,13
D7S820: 8,10
THO1: 6,7
TPOX: 8,11
vWA: 15,17

pożywka hodowlana

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Maintain cultures between 2-9x100,000 cells/ml; 5% CO₂; 37°C. Will increase 12 fold within 10-14 days provided fresh medium is added at 48-72 hour intervals. Cell growth is slow on recovery from frozen. Resuscitate using 20% FBS and keep culture flask standing up until exponential growth is established.

Inne uwagi

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