293T cell line human

12022001, human kidney, fetal. Small slightly rounded cells, dispersed growth

• Synonim(y): 293T cell line human, 293 T, 293tsA1609neo, 293tsA1609neo Cells, HEK-293-T Cells, HEK-293T Cells, HEK293T Cells, Human Embryonic Kidney 293T Cells
• Kod UNSPSC: 41106514

Właściwości

• product name: 293T cell line human, 12022001
• pochodzenie biologiczne: human kidney (fetal)
• tryb wzrostu: adherent
• morfologia: small slightly rounded cells growing in a dispersed manner
• produkty: SV40 T antigen
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Opis ogólny

293T cell line is a stable clone derivative of the human embryonic kidney (HEK) 293 cell line. It expresses the large T antigen of simian virus 40 (SV40).

Opis linii komórkowej

The 293T cell line was created in the laboratory of Michele Calos by transfection of a sub-line of adenovirus-immortalized human embryonic kidney cells with a gene encoding the SV40T-antigen and a neomycin resistance gene. The cell line is competent for replication of vectors carrying the SV40 origin of replication. The line also has favourable tissue culture, transfection, DNA replication, gene expression, and protein production properties. It gives high titres when used to produce many viral vectors such as oncoretroviruses and lentiviruses.

Zastosowanie

293T cell line human has been used:

• for lentiviral particle production
• in the expression of eukaryotic proteins
• for small interfering RNA (siRNA) transfection
• for human immunodeficiency virus (HIV) transfection for pseudoviruses production
• as a host for the transfection of pLKO.1.-TRC-ACE2 plasmid to study the localization and role of NL63-N, a nucleocapsid protein of human coronavirus NL63, in cell cycle progression

Działania biochem./fizjol.

Due to the presence of T antigen, 293T cell line favors simian virus 40 (SV40) plasmid replication as well as high protein expression. It also possesses stem cell properties. HEK293T is used for large-scale vector production, displays increased cell growth and is efficient for transfection experiments when compared to HEK293 cells.

Profil DNA

STR-PCR Data:

Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11
THO1: 7,9.3
TPOX: 11
vWA: 16,19

pożywka hodowlana

DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2-4 x 10⁴ cells/cm².

Inne uwagi

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