108CC5

8062521, Mouse x Rat Hybridoma nerve, Neuronal

• Kod UNSPSC: 41106514
• pochodzenie biologiczne: Mouse x Rat Hybridoma nerve
• tryb wzrostu: Adherent
• kariotyp: Modal No. varied with passage see reference
• morfologia: Neuronal
• produkty: Long processes after treatment with dibutyryl cAMP, dense core vesicles, excitable membranes, neurotransmitter enzymes, synthesis of neurohormones and uptake of catecholamines.
• receptory: Receptors for neurohormones: delta opioid, PGE 1 vasoactive intestinal polypeptide, adenosine, somatostatin and acetylcholine.
• powiązane choroby: cancer

Właściwości

• Nazwa produktu: 108CC5, 08062521
• pochodzenie biologiczne: Mouse x Rat Hybridoma nerve
• tryb wzrostu: Adherent
• kariotyp: Modal No. varied with passage see reference
• morfologia: Neuronal
• produkty: Long processes after treatment with dibutyryl cAMP, dense core vesicles, excitable membranes, neurotransmitter enzymes, synthesis of neurohormones and uptake of catecholamines.
• receptory: Receptors for neurohormones: delta opioid, PGE 1 vasoactive intestinal polypeptide, adenosine, somatostatin and acetylcholine.
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Mouse neuroblastoma x Rat glioma hybrid

Opis linii komórkowej

The cell line108CC5 is formed by the fusion of mouse neuroblastoma N18TG2 and rat glioma C6-BU-1using inactivated Sendai virus. This cell line was renamed NG108-5 by Klee and Nirenberg . This is a cholinergic hybrid cell line.

Zastosowanie

Used as a neuronal Model

pożywka hodowlana

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured inbetween in HT medium for 3-4 days

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required.

Inne uwagi

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Ta strona może zawierać tekst przetłumaczony maszynowo.