KARPAS 299

6072604, human lymph, Lymphoblastoid

• Synonim(y): K299 Cells, KARPAS299 Cells
• Kod UNSPSC: 41106514

Właściwości

• product name: KARPAS 299, 06072604
• pochodzenie biologiczne: human lymph
• tryb wzrostu: Suspension
• kariotyp: Hypodiploid
• morfologia: Lymphoblastoid
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Non-Hodgkin′s Ki-positive Large Cell Lymphoma

Opis linii komórkowej

A human T cell lymphoma cell line established from the peripheral blood of a 25-year-old man with T cell non-Hodgkin′s lymphoma in 1986.

Profil DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8,12
D16S539: 12
D5S818: 11,12
D7S820: 10,11
THO1: 7,8.3
TPOX: 8
vWA: 17,19

pożywka hodowlana

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). For slower growth use 10% or 5% FBS.

Rutyna subkultury

Maintain cultures between 0.5 - 2 x1000,000 cells/ml; 5% CO₂; 37°C. Doubling time approximately 30 hours. Split saturated culture 1:3 every 2-3 days. Round to polygonal cells growing singly or in small clusters in suspension.

Inne uwagi

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Oświadczenie o zrzeczeniu się odpowiedzialności

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

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