VP303

5092806, human breast, Epithelial

• Kod UNSPSC: 41106514

Właściwości

• product name: VP303, 05092806
• pochodzenie biologiczne: human breast
• tryb wzrostu: Adherent
• kariotyp: Mean no. 64, modal no. 67, range 57-72
• morfologia: Epithelial
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Breast Cancer

Opis linii komórkowej

Derived from a breast cancer biopsy removed from a 69 year-old female. The histology of the breast cancer biopsy was defined as Ductal Grade II, 90% of tumour cells stained positive for oestrogen receptors and cells were negative for progesterone receptor staining. The VP303 cell line derived from the tumour did not stain positive for oestrogen receptors. The individual was recorded to be alive 5 years after surgery performed to remove the tumour. This cell line grows slowly and is relatively difficult to culture; please read the sub-culture routine information closely.

pożywka hodowlana

MEBM (Clonetics, CC-3151) + MEGM SingleQuots (Clonetics, CC-4136) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS). Fresh EGF (final concentration of 10ng/ml) should be added to the medium at each subculture.

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. The VP series of breast cancer cell lines are very slow growing and relatively difficult to grow. Fresh EGF (final concentration 10ng/ml) s

Inne uwagi

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