CAS9ZEOVP

Cas9 Zeo Lenti Plasmid

• Kod UNSPSC: 41106609
• NACRES: NA.51

Właściwości

• rekombinowane: expressed in E. coli
• Postać: liquid
• opakowanie: vial of 50 μL
• stężenie: 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
• Zastosowanie: CRISPR
• selection: blasticidin
• Warunki transportu: dry ice
• temp. przechowywania: −20°C

Opis

Opis ogólny

This product is a lentiviral plasmid that utilizes the EF1 alpha promoter to drive expression of Cas9 and a Zeo resistance cassette linked by a 2A peptide (EF1a-Cas9-2A-Zeo) allowing for easy visualization of successful transfection or transduction. Use Sigma′s lentiviral Cas9 plasmid for generation of lentiviral particles and efficient production of stable cell lines expressing Cas9 for CRISPR based genome editing. Sigma′s Cas9-Zeo lenti plasmid is one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors.

To order gRNA in any format click here

Zastosowanie

Functional Genomics/Target Validation

• Creation of gene knockouts in multiple cell lines
• Complete knockout of genes not amenable to RNAi
• Manufacture of Cas9 expressing Lentiviral Particles

Cechy i korzyści

• Highly specific
• Highly active
• Ready to use purified plasmid DNA

Zasada

CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.

Informacje prawne

CRISPR Label License

Lentiviral, WPRE, and Evrogen Licenses

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable