C9301

Chicken Collagen Type II

from chicken sternal cartilage, powder, suitable for cell culture

• Numer CAS: 9007-34-5
• Numer WE: 232-697-4
• Numer MDL: MFCD00130825
• Kod UNSPSC: 12352202
• NACRES: NA.75

Właściwości

• Nazwa produktu: Collagen from chicken sternal cartilage, Type II (Miller), powder, BioReagent, suitable for cell culture
• pochodzenie biologiczne: chicken (Sternal cartilage)
• Poziom jakości: 300
• typ: Type II (Miller)
• linia produktu: BioReagent
• Formularz: powder
• opakowanie: glass bottle of 100 mg; poly bottle of 25 mg; glass bottle of 5 mg
• stężenie: 60-80% (biuret)
• metody: cell culture | mammalian: suitable
• pokrycie powierzchni: 6‑10 μg/cm²
• rozpuszczalność: acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)
• numer dostępu UniProt: P02460
• Specyficzność wiązania: Peptide Source: Fibrinogen
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C
• informacje o genach: chicken ... COL2A1(395069)

Opis

Opis ogólny

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Collagen is made of three polypeptide chains. Type 2 collagen is ubiquitously present in the hyaline cartilage. It is a homotrimer made of three α1 chains. Type 2 collagen is also predominant in nucleus pulposus of the vertebral disc and vitreous body of the eye. The proteoglycan obtained from the chicken sternal cartilage is similar to type nine collagen structure.

Zastosowanie

Collagen from chicken sternal cartilage has been used:

• in enzyme–linked immunosorbent assay (ELISA).
• chondrocyte-mediated tissue production in vitro.
• induction, treatment, and assessment of collagenα induced arthritis (CIA).
• cell proliferation assay.
• as a coating for cell culture surfaces.

Działania biochem./fizjol.

Collagen breaks down metabolically in the body to release N-telopeptide, which is the N-terminus of collagen. There is also C-telopeptide, which is presumably the C-terminus. N-telopeptide is released in urine, and its detection in diagnostic tests is used to screen for osteoporosis.

Cechy i korzyści

This collagen has been tested in culture with mammalian cells to verify it is low in endotoxin content.

Inne uwagi

Major component of articular cartilage.

Uwaga dotycząca przygotowania

Prepared by a modification of the method of Trentham, D.E., et al.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)