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Kluczowe dokumenty
C4407
Human Collagen Type III
from human placenta, powder, suitable for cell culture
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About This Item
Polecane produkty
product name
Collagen from human placenta, Bornstein and Traub Type III (Sigma Type X), powder
pochodzenie biologiczne
human placenta
Poziom jakości
Próba
≥90% (GE)
Postać
powder
metody
cell culture | mammalian: suitable
zanieczyszczenia
HIV, hepatitis B and hepatitis C, none detected
rozpuszczalność
aqueous acid: soluble
numer dostępu UniProt
temp. przechowywania
2-8°C
informacje o genach
human ... COL3A1(1300)
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Zastosowanie
Collagen from human placenta has been used to assess the bioelectric effects of quinine on airway epithelial cells, to study the selective toxicity of engineered lentvirus lytic peptides and in a particle aggregation assay for the rapid detection of fibronectin, fibrinogen and collagen receptors on Staphylococcus aureus. It has also been used in a magnesium-dependent, collagen-binding assay during characterization of human lung tumor-associated antigens.
Komponenty
All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue.
Przestroga
This product should be stored at 2-8 °C and will retain activity in these conditions for 2 years.
Uwaga dotycząca przygotowania
This product was prepared by a modification of the pepsin extraction and salt fractionation method of Hill, R.J. and Harper, E., Anal. Biochem., 141 (1984). It is a lyophilized powder that can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C.
Komentarz do analizy
An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.
Inne uwagi
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
Certyfikaty analizy (CoA)
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