B8503

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

chromogenic, ≥99% (HPLC), powder

• Synonim(y): 5-Bromo-4-chloro-3-indoxyl phosphate p-toluidine salt, BCIP^® p-toluidine salt, X-phosphate p-toluidine salt
• Wzór empiryczny (zapis Hilla): C₈H₆BrClNO₄P · C₇H₉N
• Numer CAS: 6578-06-9
• Masa cząsteczkowa: 433.62
• Numer WE: 229-506-1
• Numer MDL: MFCD00040636
• Kod UNSPSC: 12352204
• Identyfikator substancji w PubChem: 57653963
• NACRES: NA.83

Właściwości

• product name: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, ≥99% (HPLC)
• Próba: ≥99% (HPLC)
• Postać: powder
• rozpuszczalność: DMF: 20 mg/mL, clear to slightly hazy, yellow
• temp. przechowywania: −20°C
• ciąg SMILES: Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
• InChI: 1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
• Klucz InChI: QEIFSLUFHRCVQL-UHFFFAOYSA-N

Opis

Opis ogólny

5-bromo-4-chloro-3-indolyl phosphate (BCIP) is an histochemical phosphatase stain.

Zastosowanie

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt has been used in in situ hybridization.

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

Działania biochem./fizjol.

5-bromo-4-chloro-3-indolyl phosphate (BCIP) can be used to visualize the phosphatase activity. It can be used as a substrate to monitor secreted embryonic alkaline phosphatase activity in solution.

Opakowanie

Bottomless glass bottle. Contents are inside inserted fused cone.

Substraty

Histochemical substrate for alkaline phosphatase.

Informacje prawne

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

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