B5388

Brilliant Cresyl Blue ALD

Dye content, ≥60%, certified by the Biological Stain Commission, powder

• Synonim(y): 3-Amino-7-(diethylamino)-2-methylphenoxazin-5-ium (T-4)-tetrachlorozincate (2:1), Brilliant Blue C, Brilliant Cresyl blue
• Wzór liniowy: C₁₇H₂₀N₃OCl · 1/2ZnCl₂
• Numer CAS: 51716-96-2
• Masa cząsteczkowa: 385.96
• Numer w indeksie barw: 51010
• Beilstein: 5690713
• Numer WE: 279-675-0
• Numer MDL: MFCD00148901
• Kod UNSPSC: 12171500
• Identyfikator substancji w PubChem: 24891836
• NACRES: NA.47

Właściwości

• Nazwa produktu: Brilliant Cresyl Blue ALD, certified by the Biological Stain Commission
• klasa czystości: certified by the Biological Stain Commission
• Poziom jakości: 200
• Formularz: powder
• skład: Dye content, ≥60%
• rozpuszczalność: water: 1 mg/mL, blue to very deep blue
• Zastosowanie: diagnostic assay manufacturing; hematology; histology
• temp. przechowywania: room temp
• InChI: 1S/2C17H20N3O.4ClH.Zn/c2*1-4-20(5-2)12-6-7-14-16(9-12)21-17-10-13(18)11(3)8-15(17)19-14;;;;;/h2*6-10H,4-5,18H2,1-3H3;4*1H;/q2*+1;;;;;+2/p-4
• Klucz InChI: SIHASHFMDFXGSX-UHFFFAOYSA-J

Opis

Zastosowanie

Brilliant Cresyl Blue ALD has been used for the selection of oocytes. It has also been used for the selection of cumulus-oocyte complexes (COCs).

Działania biochem./fizjol.

Brilliant Cresyl Blue ALD is mainly used for the selection of oocytes. It helps in determining the activity of glucose-6-phosphate dehydrogenase (G6PD). This enzyme is generated in growing oocytes. However, oocytes which complete their growth have low levels of G6PD. Low G6PD activity results in blue coloration of the cytoplasm because brilliant cresyl blue is not reduced to a colourless compound. It is also used for the staining in reticulocytes, where the dye stains reticulin.

Przydatność

Certified as a supravital stain for reticulocytes.

Inne uwagi

The structure of Brilliant Cresyl Blue ALD contains a 2-methyl modification on the phenoxazine core, while the structure of the historic Brilliant Cresyl Blue contains a 8-methyl modification on the phenoxazine core. The biological staining application is the same for both structures.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Gloves