W0625

Wright stain

≤4% purity (HPLC), powder

• Synonim(y): Eosin Methylene blue according to Wright
• Numer CAS: 68988-92-1
• Numer MDL: MFCD00082143
• Kod UNSPSC: 12171500
• NACRES: NA.47

Właściwości

• product name: Wright stain, suitable for blood stain
• Próba: ≤4% (HPLC)
• Poziom jakości: 200
• Postać: powder
• stężenie: ≤4% (HPLC)
• metody: microbe id | staining: suitable
• kolor: dark green
• rozpuszczalność: methanol: 10 mg/10 mL
• przydatność: suitable for blood stain
• Zastosowanie: diagnostic assay manufacturing; hematology; histology
• temp. przechowywania: room temp
• InChI: 1S/C20H8Br4O5.C16H18N3S/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13/h1-6H,(H2-,25,26,27,28);5-10H,1-4H3/q;+1/p+1
• Klucz InChI: AXIKDPDWFVPGOD-UHFFFAOYSA-O

Opis

Opis ogólny

Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.

Zastosowanie

Wright′s Stain is mainly used for the staining of peripheral blood smears and bone marrow smears.

• It is also used for G-banding and helps in the analysis of poorly spread metaphases.
• It has been used to study the cytogenetics of human hematopoietic cells.
• It has also been employed in the karyotyping of human embryonic stem cells.
• It is also used in the staining of chromosomes.
• It is used for the exudate analysis of white blood cell concentration.

Reported to be superior to May-Grunwald Stain in blood staining.

Cechy i korzyści

The purified dyes in Wright′s Stain eliminate inconsistent staining and yield reproducible lot-to-lot chromogenic responses.

Uwaga dotycząca przygotowania

Prepared using polychromed (oxidized) methylene blue and eosin Y.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS07
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H302,H319
• Zwroty wskazujące środki ostrożności: P280 - P301 + P312 + P330 - P305 + P351 + P338 - P337 + P313
• Klasyfikacja zagrożeń: Acute Tox. 4 Oral - Eye Irrit. 2
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Gloves