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Merck

G5637

Sigma-Aldrich

Giemsa stain

certified by the Biological Stain Commission, powder or crystals

Synonim(y):

Azure mixture sicc. Giemsa stain

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About This Item

Wzór empiryczny (zapis Hilla):
C14H14ClN3S
Numer CAS:
Masa cząsteczkowa:
291.80
Numer MDL:
Kod UNSPSC:
12171500
Identyfikator substancji w PubChem:
NACRES:
NA.47

product name

Giemsa stain, certified by the Biological Stain Commission

klasa czystości

certified by the Biological Stain Commission

Poziom jakości

Postać

powder or crystals

metody

microbe id | staining: suitable

kolor

dark green to very dark green

mp

300 °C (lit.)

rozpuszczalność

ethanol: 1 mg/mL, blue to very deep blue

absorpcja

≥0.6 at 647-653 nm in methanol at 0.005 g/L

Zastosowanie

diagnostic assay manufacturing
hematology
histology

temp. przechowywania

room temp

ciąg SMILES

[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

Klucz InChI

NALREUIWICQLPS-UHFFFAOYSA-N

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Opis ogólny

Giemsa is a versatile polychromatic stain, which is suitable for staining a diverse range of specimens. It is a buffered thiazine-eosinate solution designed to provide coloration of blood cells. Giemsa’s stain is frequently used for diagnostic purposes in hematology to differentiate the nuclear and cytoplasmic morphology of platelets, RBCs, WBCs, and parasites. It is frequently used in combination with other dye solutions as May-Grunwald’s solution for Pappenheim (MGG) and Wright-Giemsa. The Giemsa stain was adapted to histology due to its unique staining of chromatin, nuclear membranes, and cytoplasmic elements. Giemsa′s staining solution is composed of methylene blue, azure, and eosin.

Zastosowanie

Giemsa stain has been used for the staining of cells and blood films.It is required for the staining of azurophil granules and is an excellent stain for inclusion bodies.Its applications can be seen in hematology for blood and bone marrow specimens, bacteriology, clinical cytology specimens, histological biopsies, and tumor samples.

Przydatność

Certified for Giemsa stain for blood films and for the Wolbach′s Giemsa method for paraffin sections.

Inne uwagi

Nuclei will be varying shades of purple. Cytoplasmic staining will be varying shades of blue to light pink. Fine reddish to lilac granules may be present in cytoplasm of some cell types. Basophils will demonstrate dark blue black granules in the cytoplasm. Eosinophils will demonstrate bright orange granules in the cytoplasm. Red blood cells should be pink to orange
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

dust mask type N95 (US), Eyeshields, Gloves


Certyfikaty analizy (CoA)

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