A9853
Anti-Androgen Receptor antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonim(y):
Androgen Receptor Antibody, Androgen Receptor Antibody - Anti-Androgen Receptor antibody produced in rabbit, Anti-AR
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About This Item
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
białko sprzężone
unconjugated
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
Postać
buffered aqueous solution
masa cząsteczkowa
antigen 110 kDa (additional band may be observed at 45 kDa)
reaktywność gatunkowa
rat, human
metody
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL using microwave-pretreated human prostate tissue sections
immunoprecipitation (IP): 5-10 μg using human MCF7 cell lysate ( 250 μg)
microarray: suitable
western blot: 1-2 μg/mL using whole extract of rat brain
numer dostępu UniProt
Warunki transportu
dry ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
Powiązane kategorie
Opis ogólny
Androgens are the male sex hormones that exert biological and transcriptional effects on binding to nuclear androgen receptor (AR). Androgens in humans include androstenedione, testosterone, dihydrotestosterone and dehydroepiandrosterone. Androgen receptor is a ligand inducible nuclear receptor, expressed on brain, bone, skeletal muscles, genital organs, hair follicles and cardiovascular tissue. On binding androgen, the AR is internalized and modulates the transcriptional of target genes. Additionally, AR also exerts extra-nuclear actions wherein it rapidly induces signalling cascades of MAPK and protein kinase C. The regulation by AR results in irreversible sexual differentiation of sex organs during embryogenesis and development of sex-specific characteristics during puberty
Anti-Androgen Receptor specifically recognizes the human androgen receptor protein (110 kDa). An additional band may be observed at 45 kDa. The antibody cross-reacts with rat androgen receptor.
Anti-Androgen Receptor specifically recognizes the human androgen receptor protein (110 kDa). An additional band may be observed at 45 kDa. The antibody cross-reacts with rat androgen receptor.
Specyficzność
The antibody cross-reacts with androgen receptor from rat.
Immunogen
evolutionally conserved peptide corresponding to amino acids 1-21 of the human androgen receptor. This sequence is not found in estrogen, progesterone, or glucocorticoid receptors.
Zastosowanie
Anti-androgen receptor antibody may be used for immunoblotting using whole rat brain extract at a recommended concentration of 1-2 μg/ml. For immunoprecipitaion from 250 μg of human MCF7 cell lysate, a concentration of 5-10 μg may be used. Immunohistochemistry may be performed on paraffin-embedded human prostate tissue sections at a working antibody concentration of 20-40 μg/ml.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Rabbit anti-androgen receptor (C19) was used in a EMSA (electrophoretic mobility shift assay) which produced a supershift in the molecular weight of a particular DNA sequence indicating binding of the androgen receptor.
Postać fizyczna
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certyfikaty analizy (CoA)
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