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Merck

A7721

Sigma-Aldrich

JumpStart Taq Antibody

Adds hot-start capabilities to any Taq DNA Polymerase

Synonim(y):

Hot start Taq antibody

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About This Item

Numer MDL:
Kod UNSPSC:
41106300
NACRES:
NA.55

pochodzenie biologiczne

mouse

białko sprzężone

unconjugated

klon

monoclonal

Postać

liquid

Właściwości

hotstart

stężenie

1.1 mg/mL

metody

PCR: suitable

kolor

colorless

Warunki transportu

wet ice

temp. przechowywania

−20°C

Opis ogólny

JumpStart Taq Antibody provides an efficient method for hot-start PCR. This antibody is a neutralizing monoclonal antibody specific to Taq DNA polymerase. When the antibody is bound to Taq, the enzyme is rendered inactive. At the first denaturation step in thermal cycling, the enzyme-antibody complex dissociates, and the JumpStart Taq Antibody becomes nonfunctional. Once the antibody is denatured, the activity of the Taq DNA polymerase is restored and the enzyme functions normally during the PCR reaction. JumpStart Taq Antibody is commonly used to prevent undesirable amplification products.

Zastosowanie

JumpStartTaq Antibody is suitable for use in:
  • cytochrome blocked nucleic acid (LNA) probe assay
  • ligation-mediated polymerase chain reaction (LM-PCR)
  • in Real-Time quantitative PCR Analysis of viral titer
  • PCR amplification of complex genomic or cDNA templates
  • to amplify very low-copy-number targets
  • for the PCR amplification of many thermal cycles (>35) and multiple primer pairs in the same reaction tube

Opakowanie

Supplied with dilution buffer.

Komponenty

JumpStart Taq Antibody, 1.1μg/1μL
Dilution Buffer provided in 1 mL vials

Ilość

Two units of Taq DNA polymerase are inactivated by 1 test of JumpStart Taq Antibody.

Inne uwagi

JumpStart Taq Antibody is for R&D use only.Not for drug, household, or other uses.

View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.

Informacje prawne

JumpStart is a trademark of Sigma-Aldrich Co. LLC
This page may contain text that has been machine translated.

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

D J Sharkey et al.
Bio/technology (Nature Publishing Company), 12(5), 506-509 (1994-05-01)
We demonstrate the utility of antibodies to the DNA polymerase from Thermus aquaticus (TaqPol) as thermolabile inhibitors of TaqPol activity. One of the limitations of the polymerase chain reaction (PCR) is the co-amplification of non-specific products caused by TaqPol activity
Enhanced gene targeting of adult and pluripotent stem cells using evolved adeno-associated virus
Gene correction, 169-179 (2014)
Measuring and monitoring apoptosis and drug toxicity in HIV patients by ligation-mediated polymerase chain reaction
Hooker DJ, et al.
Journal of Cellular and Molecular Medicine, 13(5), 948-958 (2009)
Application of real-time and multiplex polymerase chain reaction assays to study leaf blotch epidemics in barley
Fountaine JM, et al.
Phytopathology, 97(3), 297-303 (2007)

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