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Key Documents
A3983
Bovine Serum Albumin
lyophilized powder, essentially γ-globulin free, ≥98% (agarose gel electrophoresis)
Synonim(y):
Albumin bovine serum, BSA, Bovine albumin
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About This Item
Polecane produkty
pochodzenie biologiczne
bovine
Poziom jakości
Próba
≥98% (agarose gel electrophoresis)
Postać
lyophilized powder
masa cząsteczkowa
~66 kDa
oczyszczone przez
heat shock fractionation
opakowanie
poly bottle of
pochodzenie
New Zealand origin
zanieczyszczenia
essentially globulin free
strata
≤5%
pH
7
rozpuszczalność
water: soluble, clear to slightly hazy (1 g + 25 mL)
numer dostępu UniProt
obecność zanieczyszczeń
Protease free
temp. przechowywania
2-8°C
informacje o genach
bovine ... ALB(280717)
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Opis ogólny
Bovine Serum Albumin has three homologous domains (I, II, and III) that are connected by disulfide bonds. BSA shares structural homology with human serum albumin (HSA).
Zastosowanie
Bovine Serum Albumin has been used:
- as a component of blocking solution used for fixing Drosophila embryos in immunostaining
- to block 96-deep-well plates in phage immunoprecipitation studies
- as a standard solution in fluorescence spectroscopy studies
Działania biochem./fizjol.
Bovine Serum Albumin (BSA) displays intrinsic fluorescence due to the presence of two tryptophan residues. It is widely used in ligand interaction studies and in the delivery of drugs and antibodies. BSA is used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Uwaga dotycząca przygotowania
Derived from New Zealand source serum
Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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