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Merck

A3733

Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, ≥98% (agarose gel electrophoresis)

Synonim(y):

Albumin bovine serum, BSA, Bovine albumin

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About This Item

Numer CAS:
Numer WE:
Numer MDL:
Kod UNSPSC:
12352202
NACRES:
NA.27

pochodzenie biologiczne

bovine

Poziom jakości

Próba

≥98% (agarose gel electrophoresis)

Postać

lyophilized powder

masa cząsteczkowa

~66 kDa

oczyszczone przez

heat shock fractionation

opakowanie

poly bottle of

pochodzenie

New Zealand origin

metody

flow cytometry: suitable

pH

7
~7 (1% in 0.15 M NaCl)

rozpuszczalność

water: slightly soluble

numer dostępu UniProt

temp. przechowywania

2-8°C

informacje o genach

bovine ... ALB(280717)

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Opis ogólny

Bovine serum albumin (BSA) that belongs to the serum albumin family, is an α-helical, globular and non-glycosylated protein. It comprises three domains with two subdomains under each domain.

Zastosowanie

Bovine Serum Albumin has been used:
  • as a component of cryomedia for freezing testicular tissue
  • to resuspend myometrial cells in flow cytometric analysis for cell viability quantification
  • to block cardiac fibroblasts (CFs) for immunofluorescence staining

Działania biochem./fizjol.

Bovine Serum Albumin (BSA) is a transporter for drugs, hormones, and fatty acids. It displays high solubility and is exploited in the cosmetic and pharmaceutical industries. BSA is useful as a cell media component and favors embryonic stem cells (hESC)differentiation.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Uwaga dotycząca przygotowania

Derived from New Zealand source serum
Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
This page may contain text that has been machine translated.

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


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Odwiedź Bibliotekę dokumentów

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Journal of pharmaceutical sciences, 108(5), 1686-1691 (2019-01-12)
Cyclo olefin polymer (COP) is an attractive plastic because it has low protein adsorption despite its hydrophobic chemical structure. Here, the adsorption of model proteins to the COP was evaluated in comparison with a representative plastic, polystyrene (PSt), using reflectometry
Tamara Topală et al.
Clujul medical (1957), 87(4), 215-219 (2014-01-01)
The continuous search for new molecules with therapeutic abilities has led to the synthesis and characterization of a large number of metal complexes, proven to exhibit potential as pharmacological agents through their antibacterial, antiviral, antifungal and antineoplastic properties. As serum
Balázs Rada et al.
Journal of immunology (Baltimore, Md. : 1950), 181(7), 4883-4893 (2008-09-20)
The dual oxidase-thiocyanate-lactoperoxidase (Duox/SCN(-)/LPO) system generates the microbicidal oxidant hypothiocyanite in the airway surface liquid by using LPO, thiocyanate, and Duox-derived hydrogen peroxide released from the apical surface of the airway epithelium. This system is effective against several microorganisms that
Ignacio Izeddin et al.
PloS one, 6(1), e15611-e15611 (2011-01-26)
The actin cytoskeleton of dendritic spines plays a key role in morphological aspects of synaptic plasticity. The detailed analysis of the spine structure and dynamics in live neurons, however, has been hampered by the diffraction-limited resolution of conventional fluorescence microscopy.
Manoj K Pandey et al.
Molecular pharmacology, 75(3), 525-533 (2008-12-24)
The aim of the current study is to determine whether butein (3,4,2',4'-tetrahydroxychalcone) exhibits antiproliferative effects against tumor cells through suppression of the signal transducer and activator of transcription 3 (STAT3) activation pathway. We investigated the effects of butein on constitutive

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