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Merck

B6917

Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, suitable for (for molecular biology), Non-acetylated

Synonim(y):

Albumin bovine serum, BSA, Bovine albumin

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About This Item

Numer CAS:
Numer WE:
Numer MDL:
Kod UNSPSC:
12352202
NACRES:
NA.27

pochodzenie biologiczne

bovine

Poziom jakości

klasa czystości

for molecular biology

Próba

≥98% (agarose gel electrophoresis)

Postać

lyophilized powder

jakość

deacetylated

masa cząsteczkowa

~66 kDa

oczyszczone przez

heat shock fractionation

opakowanie

glass bottle of

pochodzenie

USA origin

metody

electrophoresis: suitable-98% using Agarose

zanieczyszczenia

enzymatic impurity, none detected

pH

7

rozpuszczalność

water: soluble (50 mg/ml)

przydatność

suitable for (for molecular biology)

numer dostępu UniProt

obecność zanieczyszczeń

Alkaline Phosphatase free
Alkaline phosphatase and peroxidase, none detected
DNase free
DNase, RNase, and protease, none detected
Peroxidase free
Protease free
RNase free

temp. przechowywania

2-8°C

informacje o genach

bovine ... ALB(280717)

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Opis ogólny

Bovine Serum Albumin (BSA) is a plasma protein produced in the bovine liver that has a globular, non-glycosylated α-helical structure. It consists of a single polypeptide chain with 583 amino acid residues and 17 cysteine residues that form the cross-linked disulfide bridges, stabilizing its structure. The BSA protein comprises three structurally distinct domains (I, II, and III), each of which contains two sub-domains (A and B) arranged in a heart-shaped architecture.

Zastosowanie

Bovine Serum Albumin (BSA) has been used
  • as a component of phosphate buffer saline (PBS) solution for dissolving tumor necrosis factor α (TNFα), which was added as a supplement for culture media
  • as a blocking agent to reduce non-specific binding during the albumin sensor assay
  • as a blocking agent in the immunofluorescence (IF) assay
  • as a protein standard in the Bradford assay

Działania biochem./fizjol.

Bovine Serum Albumin (BSA) is a versatile protein commonly utilized in cell and tissue culture media as a supplement. It helps in lipid solubilization and stabilizing other proteins. BSA is also a carrier for small molecules such as steroids, fatty acids, and thyroid hormones. It serves as a standard protein for immunochemical tests to determine protein concentration in a solution and calibration studies.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Cechy i korzyści

This BSA product for molecular biology is tested for the following properties:
* Alkaline Phosphatase-free
* DNase-free / Exonuclease-free
* Nickase-free / Endonuclease-free
* Peroxidase-free
* Protease-free
* RNase-free

Uwaga dotycząca przygotowania

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
This page may contain text that has been machine translated.

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

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Fadi Jacob et al.
Cell, 180(1), 188-204 (2019-12-31)
Glioblastomas exhibit vast inter- and intra-tumoral heterogeneity, complicating the development of effective therapeutic strategies. Current in vitro models are limited in preserving the cellular and mutational diversity of parental tumors and require a prolonged generation time. Here, we report methods for
Balázs Rada et al.
Journal of immunology (Baltimore, Md. : 1950), 181(7), 4883-4893 (2008-09-20)
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Ignacio Izeddin et al.
PloS one, 6(1), e15611-e15611 (2011-01-26)
The actin cytoskeleton of dendritic spines plays a key role in morphological aspects of synaptic plasticity. The detailed analysis of the spine structure and dynamics in live neurons, however, has been hampered by the diffraction-limited resolution of conventional fluorescence microscopy.
Manoj K Pandey et al.
Molecular pharmacology, 75(3), 525-533 (2008-12-24)
The aim of the current study is to determine whether butein (3,4,2',4'-tetrahydroxychalcone) exhibits antiproliferative effects against tumor cells through suppression of the signal transducer and activator of transcription 3 (STAT3) activation pathway. We investigated the effects of butein on constitutive

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