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Merck

TRIPURE-RO

Roche

TriPure Isolation Reagent

solution (ready-to-use), pkg of 50 mL (11667157001), pkg of 200 mL (11667165001)

Synonim(y):

DNA purification, Protein isolation, RNA isolation, nucleic acid isolation

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About This Item

Kod UNSPSC:
41105500

Postać

solution (ready-to-use)

Poziom jakości

opakowanie

pkg of 200 mL (11667165001)
pkg of 50 mL (11667157001)

producent / nazwa handlowa

Roche

temp. przechowywania

2-8°C

Opis ogólny

Monophasic solution of phenol and guanidine thiocyanate for RNA, DNA, and protein isolation.

Zastosowanie

Ready-to-use reagent for the simultaneous isolation of total RNA, DNA, and proteins from cells or tissues of human, plant, yeast, bacterial, or viral origin. RNA is undegraded and free of protein or DNA contamination according to the current Quality Control.After purification, many applications are possible:
  • The DNA-free total RNA is suitable for northern blotting, in vitro translation, RNase protection assays, cDNA synthesis, or RT-PCR.
  • The RNA-free DNA is suitable for PCR, restriction digestion, Southern blotting, or cloning.
  • The denatured protein is suitable for SDS-PAGE and western blotting.

Cechy i korzyści

The TriPure Isolation Reagent allows the simultaneous isolation of RNA, DNA, and protein from the same sample in a single-step liquid-phase separation. RNA, DNA, and protein are separated into different organic and aqueous phases, from which each can be purified by a series of alcohol precipitations.
  • Work with an easy-to-use kit. The red dye in the reagent simplifies identification of different phases.
  • Process a wide variety of starting samples. Easily adapt the kit to your specific needs.
  • Simplify isolation protocols. Use a single reagent that isolates DNA-free RNA, RNA-free DNA, and proteins.
  • Increase yields of intact RNA. TriPure Isolation Reagent provides an immediate chaotropic denaturing environment that eliminates endogenous RNase activity.

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

Informacje prawne

TriPure is a trademark of Roche
This page may contain text that has been machine translated.

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Klasyfikacja zagrożeń

Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Zagrożenia dodatkowe

Kod klasy składowania

8A - Combustible, corrosive hazardous materials

Klasa zagrożenia wodnego (WGK)

WGK 2


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Melike Dinccelik-Aslan et al.
Molecular and clinical oncology, 3(3), 584-590 (2015-07-03)
Gastric Cancer is one of the most common malignancies worldwide and the second most common cause of cancer-related mortality. Previous studies revealed several genetic alterations specific to gastric cancer. In this study, we aimed to investigate the diagnostic and prognostic
JanWillem Duitman et al.
Laboratory investigation; a journal of technical methods and pathology, 94(1), 89-97 (2013-11-20)
CCAAT-enhancer-binding protein delta (C/EBPδ) is a transcription factor mainly known for its role in inflammation and apoptosis/proliferation. Considering that these are key processes in renal fibrosis, we hypothesized that C/EBPδ would potentiate renal fibrosis. In line with this hypothesis, C/EBPδ
Hou-Zao Chen et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(36), 11897-11912 (2014-09-05)
The failure of past efforts to develop effective stroke treatments is at least partially because these treatments often interfered with essential physiological functions, even though they are targeted toward pathophysiological events, such as inflammation, excitotoxicity, and oxidative stress. Thus, the

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