10223565001
Roche
Histone
from calf thymus
Synonim(y):
histone
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352204
Poziom jakości
Postać
lyophilized
opakowanie
pkg of 10 mg
producent / nazwa handlowa
Roche
Warunki transportu
wet ice
temp. przechowywania
2-8°C
Powiązane kategorie
Opis ogólny
Mixture of histones H1, H2A, H2B, H3, and H4, isolated from calf thymus.
Histones are a group of DNA-binding proteins that are characterized by relatively high levels of lysine and arginine. Five different fractions of histones have been isolated and characterized. These are named as H1, H2A, H2B, H3 and H4. The H1 fraction is lysine-rich, while the H2A and H2B are slightly lysine-rich, The H3 and H4 fractions are arginine-rich. The molecular weights of histones are approximately 11 to 21 kDa depending on the fraction.
Histones are a group of DNA-binding proteins that are characterized by relatively high levels of lysine and arginine. Five different fractions of histones have been isolated and characterized. These are named as H1, H2A, H2B, H3 and H4. The H1 fraction is lysine-rich, while the H2A and H2B are slightly lysine-rich, The H3 and H4 fractions are arginine-rich. The molecular weights of histones are approximately 11 to 21 kDa depending on the fraction.
Zastosowanie
Histone from calf thymus has been used:
- in in vitro kinase assay to check the phosphorylation of histones by protein kinase A.
- to check the in vitro methyltransferase activity of protein argininemethyltransferases from Oryza sativa (OsPRMTs).
- in anti-histone ELISA.
Działania biochem./fizjol.
Histones have an important role in the organization and modification of chromatin. The nucleosome is the basic unit of the chromatin. It is made up of two molecules each of histones H2A, H2B, H3 and H4, wrapped with approximately 146bp of DNA. Histone H1 binds to the nucleosomes at the DNA entry and exit points and stabilizes them.
Postać fizyczna
Lyophilizate (natural mixture of histones H1, H2A, H2B, H3, and H4).
Uwaga dotycząca przygotowania
Working concentration: 1 to 5 mg/ml.
Working solution: Recommended solvent is double-distilled water.
Storage conditions (working solution): Aqueous solution is stable at -15 to -25 °C for several months.
Avoid repeated freezing and thawing!
Working solution: Recommended solvent is double-distilled water.
Storage conditions (working solution): Aqueous solution is stable at -15 to -25 °C for several months.
Avoid repeated freezing and thawing!
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
This page may contain text that has been machine translated.
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
H1 histone and the condensation of chromatin and DNA.
R D Cole et al.
Cold Spring Harbor symposia on quantitative biology, 42 Pt 1, 253-263 (1978-01-01)
Core histones H2B and H4 are mobilized during infection with herpes simplex virus 1.
Conn KL, et.al
Journal of Virology, 85(24), 13234-13252 (2011)
Ona Bloom et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(25), 10255-10259 (2011-06-08)
Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by the presence of pathogenic autoantibodies, many of which are directed against nuclear antigens, in particular double-stranded (ds) DNA. Both clinical studies and animal models have shown that anti-dsDNA antibodies
Abinaya Rajendran et al.
Biochemistry, 59(2), 183-196 (2019-09-04)
The metabolic serine hydrolase family is, arguably, one of the largest functional enzyme classes in mammals, including humans, comprising 1-2% of the total proteome. This enzyme family uses a conserved nucleophilic serine residue in the active site to perform diverse
The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly.
Ahmad K and Henikoff S
Molecular Cell, 9(6) (2002)
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