About This Item
Polecane produkty
pochodzenie biologiczne
bovine (calf) thymus
Poziom jakości
typ
Type III-S
Postać
powder
stężenie
≥60% protein (biuret)
metody
activity assay: suitable
rozpuszczalność
H2O: soluble 10 mg/mL, clear to hazy, colorless to light yellow
temp. przechowywania
2-8°C
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Zastosowanie
Histone is suitable for use:
- as a positive control in the in vitro kinase assay to examine the ability of Cdc2-cyclin B1 to phosphorylate GST-CD-4 protein
- in Cdk5 activity assay
- in histone H1 kinase assay
- as a Poly(ADP-ribose) (PAR) acceptor in the assessment of in vitro PAR polymerase activity
- as an acceptor of 32P from [γ-32P]ATP in order to measure protein kinase activity of lysate from small and large luteal cells
Działania biochem./fizjol.
Histones are a group of proteins characterized by high levels of lysine and arginine that form reversible complexes with DNA. Based on the fractions, the molar mass of histones varies between 11-21kDa. Five different fractions have been isolated and characterized. They include H1 (lysine rich), H2a and H2b that are slightly lysine rich, H3 and H4 that are arginine rich. H1 acts as a link between "beads" (nu bodies) on the chromatin chain.
Cechy i korzyści
Do not confuse Sigma "Type" designations with the "Fraction" designations used by Luck and co-workers.
Inne uwagi
Characterized as mainly subgroup f1 by SDS-PAGE
Uwaga dotycząca przygotowania
Lysine-rich fraction as isolated and described by de Nooij, E. and Westenbrink, H.G., Biochim. Biophys. Acta, 62, 608 (1962).
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
Certyfikaty analizy (CoA)
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