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10109878001

Roche

Trypsin Inhibitor

from chicken egg white

Synonim(y):

trypisin

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About This Item

Kod UNSPSC:
12352207

pochodzenie biologiczne

chicken egg white

Poziom jakości

100

Postać

powder

opakowanie

pkg of 1 g

producent / nazwa handlowa

Roche

stężenie

1 mg/mL

metody

cell culture | mammalian: suitable

optymalne pH

3.8-4.5

rozpuszczalność

water: soluble

temp. przechowywania

2-8°C

Opis ogólny

Egg white trypsin inhibitor is an inhibitor for trypsin, plasmin and plasma kallikrein activity in serum-free cell-culture media. It does not inhibit metallo-, cysteine, and aspartic proteases or tissue kallikrein (serine protease).

Zastosowanie

Chicken egg Trypsin Inhibitor is used for the termination of tissue disaggregation and for subcultivation procedures.
Trypsin Inhibitor has been used:
  • as a component of lysis buffer to treat Tsc2fl/fl (control) and Tsc2fl/flLyz2-Cre (knockout [KO]) bone marrow-derived macrophages (BMDMs) before immunoblotting
  • to inactivate trypsin post detachment of the stem cells in ventricular zone cell culture
  • to neutralize trypsin post dissociation and trypsinization of brain tissues
  • termination of tissue disaggregation and for subcultivation procedures

Uwaga dotycząca przygotowania

Working concentration: 0
Working solution: Solvent is recommended in distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
Stable for at least 6 months in distilled water or 1 mM HCI when stored frozen in aliquots. Avoid repeated freeze-thaw cycles.

Rekonstytucja

Both are soluble in water. Recommended stock solution: 1 mg/ml. Store frozen in aliquots at -15 to -25 °C. Stable for at least 6 months.

Przechowywanie i stabilność

Store at 2 to 8 °C. (Store dry!)

Inne uwagi

For life science research only. Not for use in diagnostic procedures.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

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Odwiedź Bibliotekę dokumentów

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Haley M Peckham et al.
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Myelination is a complex process that involves both neurons and the myelin forming glial cells, oligodendrocytes in the central nervous system (CNS) and Schwann cells in the peripheral nervous system (PNS). We use an in vitro myelination assay, an established
Membrane properties and electrogenesis in the distal axons of small dorsal root ganglion neurons in vitro.
Vasylyev DV and Waxman SG
Journal of Neurophysiology, 108(3), 729-740 (2012)
Nikesh Kunder et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 42(49), 9129-9141 (2022-10-22)
HuR is an RNA-binding protein implicated in RNA processing, stability, and translation. Previously, we examined protein synthesis in dorsal root ganglion (DRG) neurons treated with inflammatory mediators using ribosome profiling. We found that the HuR consensus binding element was enriched
Leandro Castaneyra-Ruiz et al.
Fluids and barriers of the CNS, 17(1), 46-46 (2020-07-22)
Severe intraventricular hemorrhage (IVH) is one of the most devastating neurological complications in preterm infants, with the majority suffering long-term neurological morbidity and up to 50% developing post-hemorrhagic hydrocephalus (PHH). Despite the importance of this disease, its cytopathological mechanisms are

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