10108014001

Papaina

from Carica papaya

• Synonim(y): papaina, proteaza cysteinowa
• Numer EC enzymu: 3.4.22.2 (BRENDA, IUBMB)
• Kod UNSPSC: 12352204

Właściwości

• pochodzenie biologiczne: papaya
• Poziom jakości: 100
• Formularz: suspension
• aktywność właściwa: 30 U/mg; ~30 units/mg protein (At 25 °C with BAEE as the substrate.)
• masa cząsteczkowa: 23 kDa
• opakowanie: pkg of 10 mL (100 mg)
• producent / nazwa handlowa: Roche
• stężenie: 10-100 mg/mL
• optymalne pH: 6.0-7.0
• Warunki transportu: wet ice

Opis

Opis ogólny

Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site.

Zastosowanie

Papain has been used in the digestion of various brain cells.

Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity.

Działania biochem./fizjol.

Papain exhibits proteolytic activity against amide links, amino acid esters, peptides, proteins. It mainly cleaves peptide bonds containing amino acids such as lysine, arginine and residues succeeding phenylalanine. It can catalyze several reactions, such as hydrolysis, transferase action, specificity and acyl-enzyme intermediate.

Cechy i korzyści

Inhibitors: SH-blocking reagents, iodoacetic acid, iodoacetamide, TPCK, TLCK, leupeptin, a2-macroglobulin, E-64, PMSF, and antipain, Hg2+, and other heavy metals

Postać fizyczna

Suspension, crystalline, nonsterile

Uwaga dotycząca przygotowania

Working concentration: 0.05 to 0.5 mg/ml

Przechowywanie i stabilność

Store at 2 to 8 °C. (a decrease in activity of 20% may occur within 6 months)

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): nwg
• Temperatura zapłonu (°F): does not flash
• Temperatura zapłonu (°C): does not flash