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11243217001

Roche

Laminin

from mouse Engelbreth-Holm-Swarm (EHS) sarcoma

Synonim(y):

Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane

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About This Item

Numer CAS:
114956-81-9
Numer MDL:
MFCD00081739
Kod UNSPSC:
12352207

pochodzenie biologiczne

mouse (Engelbreth-Holm-Swarm (EHS) sarcoma)

Poziom jakości

100

sterylność

sterile; sterile-filtered

Próba

≥90% (SDS-PAGE)

Postać

solution

masa cząsteczkowa

900 kDa

opakowanie

pkg of 1 mg (2 ml)

producent / nazwa handlowa

Roche

metody

cell culture | mammalian: suitable

numer dostępu UniProt

Q61292

temp. przechowywania

−20°C

informacje o genach

mouse ... Lamb2(16779)

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Opis ogólny

Laminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. Laminin is a heterotrimer made from α, β and γ chain subunits. It is a glycoprotein which is required for the structural scaffolding of basement membranes. Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells.

Biological activity: Tested for the promotion of adherence of HT-1080 cells.
Species specificity: Active on most mammalian cells.

Zastosowanie

Laminin has been demonstrated to promote the attachment and growth of a variety of cells; used for the coating of culture dishes. It has been used for cell adhesion assay. It is recommended for use as a cell culture substratum at 1-2μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.

Jakość

Purity: ≥90% (SDS-PAGE) (as laminin nidogen-complex, 1:1)

Sekwencja

Mouse laminin is composed of three polypeptide chains (A: 400 kD, B1: 230 kD and B2: 220 kD), connected by disulphide bridges, and is glycosylated.

Postać fizyczna

Solution, 0.5 mg/ml laminin in 0.15 M NaCl, 2 mM EDTA, 0.05 M Tris-HCl, pH 7.4, sterile
Solution, filtered through 0.2 ?m pore size membrane

Uwaga dotycząca przygotowania

Working concentration: 2 to 5 μg/cm2
2 to 5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): 2 to 8 °C
The undiluted solution is stable at 2 to 8 °C for at least 3 months.
The solution should be thawed carefully at 2 to 8 °C or on ice.

Inne uwagi

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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

nwg

Temperatura zapłonu (°F)

does not flash

Temperatura zapłonu (°C)

does not flash

Certyfikaty analizy (CoA)

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Odwiedź Bibliotekę dokumentów

Julie V Harness et al.
PloS one, 6(1), e14499-e14499 (2011-01-21)
As human embryonic stem cell (hESC) lines can be derived via multiple means, it is important to determine particular characteristics of individual lines that may dictate the applications to which they are best suited. The objective of this work was
H Colognato et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 218(2), 213-234 (2000-06-08)
The laminins are a family of glycoproteins that provide an integral part of the structural scaffolding of basement membranes in almost every animal tissue. Each laminin is a heterotrimer assembled from alpha, beta, and gamma chain subunits, secreted and incorporated
Anindita Dutta et al.
International journal of clinical and experimental pathology, 3(3), 288-302 (2010-03-13)
The interaction of cells with adhesion proteins in the extracellular matrix (ECM) provides signals which affect the morphology, motility, gene expression and survival of adherent cells. In the present communication we cultured K562 cells in presence of fibronectin to study
Elias Theodorou et al.
Genes & development, 23(5), 575-588 (2009-03-10)
Neuronal differentiation is a complex process that involves a plethora of regulatory steps. To identify transcription factors that influence neuronal differentiation we developed a high throughput screen using embryonic stem (ES) cells. Seven-hundred human transcription factor clones were stably introduced
Plamena R Angelova et al.
Methods in molecular biology (Clifton, N.J.), 2202, 33-42 (2020-08-29)
Production of reactive oxygen species (ROS) in the mitochondria plays multiple roles in physiology, and excessive production of ROS leads to the development of various pathologies. ROS in the mitochondria are generated by various enzymes, mainly in the electron transporvt
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