T2327

Trypsin inhibitor

lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)

• Synonim(y): Kunitz Inhibitor
• Numer CAS: 9035-81-8
• Numer WE: 232-906-9
• Numer MDL: MFCD00082102
• Kod UNSPSC: 12352204
• NACRES: NA.77

Właściwości

• product name: Trypsin Inhibitor from Glycine max (soybean), BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)
• pochodzenie biologiczne: Glycine max (soybean)
• Poziom jakości: 200
• linia produktu: BioUltra
• Próba: ≥95% (Kunitz inhibitor, SDS-PAGE)
• Postać: lyophilized powder
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Trypsin Inhibitor from Glycine max (soybean) also known as Kunitz trypsin inhibitor is a 21 kDa protein with a single trypsin binding reactive site.

Zastosowanie

Trypsin Inhibitor from Glycine max (soybean) has been used:

• as a standard protein to measure the amount of endogenous trypsin inhibitor present in midgut lysate (M1) of Riptortus pedestris
• as a standard to compare the trypsin inhibitory activity of the purified protein
• to monitor the trypsin inhibitory activity by fractionating in MonoS cation exchange chromatography
• as an trypsin inhibitor

Działania biochem./fizjol.

Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yield the modified form or the native inhibitor. At the optimal pH for trypsin binding (pH 8.0), the association constant is ≥ 10x10⁸.

Trypsin Inhibitor from Glycine max (soybean) binds with the active site of trypsin enzyme, in a competitive inhibition manner.

Definicja jednostki

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.

Uwaga dotycząca przygotowania

Further purification of T9128 yielding an electrophoretically pure Kunitz inhibitor with increased activity.

Trypsin inhibitor is soluble in water and phosphate buffers at concentrations of 10 mg/ml or higher. Solutions at higher concentrations may be hazy and have a yellow to amber color.

Komentarz do analizy

One mg will inhibit ≥1.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Inne uwagi

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H317,H334
• Zwroty wskazujące środki ostrożności: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Klasyfikacja zagrożeń: Resp. Sens. 1 - Skin Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)