796-0657

HPLC Analysis of Proteins on Discovery^® BIO PolyMA-WAX (Analyte Set #1)

application for HPLC

Właściwości

• opis: Separated on Discovery^® BIO PolyMA-WAX
• metody: HPLC: suitable
• parametry testowe: column: Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U); mobile phase: [A] 10 mM Tris, pH 8.0 with HCl; [B] 10 mM Tris, 0.5 M NaCl, pH 8.0 with HCl; 5 to 100% B in 15 min. (linear); flow rate: 0.5 mL/min; detector: UV, 280 nm; injection: 10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A
• przydatność: application for HPLC
• Zastosowanie: life science and biopharma

Opis

Komentarz do analizy

The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the separation of proteins that vary from 18 to 80 kDA.

Informacje prawne

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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Analit

Trypsin Inhibitor