Przejdź do zawartości
Merck
Wszystkie zdjęcia(1)

Key Documents

View All Documentation

RSHMAG-69K

Millipore

MILLIPLEX® Rat Stress Hormone Magnetic Bead Panel - Endocrine Multiplex Assay

This MILLIPLEX MAP Rat Stress Hormone Magnetic Bead Panel is an Endocrine multiplex assay. The analytes available for this multiplex kit are: ACTH, Corticosterone, Melatonin.

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
Wszystkie zdjęcia(1)

About This Item

Kod UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.84

Poziom jakości

200

reaktywność gatunkowa

rat

producent / nazwa handlowa

Milliplex®

assay range

accuracy: 61%
(Corticosterone)
accuracy: 83%
(ACTH)
sensitivity: 0.9 pg/mL
(ACTH)
sensitivity: 1.52 pg/mL
(Melatonin)
sensitivity: 2914 pg/mL
(Corticosterone)
standard curve range: 0.55-400 pg/mL
(Melatonin)
standard curve range: 1.4-1,000 pg/mL
(ACTH)
standard curve range: 686-500,000 pg/mL
(Corticosterone)
inter-assay cv: <15%
intra-assay cv: <10%

metody

multiplexing: suitable

metoda wykrywania

fluorometric (Luminex xMAP)

Warunki transportu

wet ice

Powiązane kategorie

Opis ogólny

Stress is defined as the disruption of homeostasis through physical and/or psychological stimuli orstressors. Physiologically the neuroendocrine system, which includes the hypothalamus, pituitary and adrenal glands, plays a critical role in response to stress. Activated by corticotropin-releasing hormone (CRH) and arginine-vasopressin (AV), the pituitary gland releases ACTH which results in the secretion of cortisol / corticosterone and other glucocorticoids from the adrenal glands. These corticoids involve the whole body in the stress response and are involved in the termination of the stress response by a negative feedback loop to the hypothalamus. Corticosterone and melatonin are important in the regulation of the sleep-wake cycle.

MILLIPLEX® Rat Stress Hormone Panel is a 3-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, tissue/cell extracts or culture media samples: ACTH, corticosterone and/or melatonin. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Endocrine

Specyficzność

Cross Reactivty
This assay display insignificant cross-reactivity within the panel

Zastosowanie

  • Analytes: ACTH, Corticosterone, Melatonin
  • Recommended Sample Type: Rat serum, plasma, cell/tissue supernatants and lysates
  • NOTE: For optimal assay performance of turbid serum and plasma samples an extraction protocol is provided.
  • Recommended Sample Dilution: 25μL per well is used for the following: undiluted extracted serum or plasma; 1:4 non-extracted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • Research Category: Endocrine
  • Research Subcategory: Metabolism

Cechy i korzyści

Design your multiplex kit by choosing available analytes within this panel.

Inne uwagi

Please contact Technical Service for linearity of dilution.

Informacje prawne

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This page may contain text that has been machine translated.

Piktogramy

Skull and crossbonesEnvironment
GHS06,GHS09

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H302 + H332,H311,H317,H319,H411

Klasyfikacja zagrożeń

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1

Kod klasy składowania

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Jorge Moreno-Fernandez et al.
Nutrients, 11(3) (2019-03-17)
Although dietary iron is a determinant of iron status in animals, body fat mass has been reported to have an inverse association with iron status in human studies. The goal of this study was to determine the relationship between Fe
Chris P Jew et al.
PloS one, 8(8), e70415-e70415 (2013-08-14)
The group I metabotropic glutamate receptor 5 (mGluR5) has been implicated in the pathology of various neurological disorders including schizophrenia, ADHD, and autism. mGluR5-dependent synaptic plasticity has been described at a variety of neural connections and its signaling has been
Brooks A Gross et al.
Journal of neuroscience methods, 251, 83-91 (2015-05-28)
Sleep deprivation via gentle handling is time-consuming and personnel-intensive. We present here an automated sleep deprivation system via air puffs. Implanted EMG and EEG electrodes were used to assess sleep/waking states in six male Sprague-Dawley rats. Blood samples were collected
Brenda B García-Iglesias et al.
Neuropharmacology, 71, 216-227 (2013-04-02)
Serotonin (5-HT) modulates the hypothalamic-pituitary-adrenal (HPA) axis response to stress. We examined the effect of chronic restraint stress (CRS; 20 min/day) as compared to control (CTRL) conditions for 14 days, on: 1) restraint-induced ACTH and corticosterone (CORT) secretion in rats
Fernando Tadeu Serra et al.
Scientific reports, 9(1), 13684-13684 (2019-09-25)
Life experiences at early ages, such as physical activity in childhood and adolescence, can result in long-lasting brain effects able to reduce future risk of brain disorders and to enhance lifelong brain functions. However, how early physical exercise promotes these
Wyświetl wszystkie powiązane dokumenty

Powiązane treści

Multipleksowanie biomarkerów zespołu metabolicznego

Wielokrotne testy immunologiczne oszczędzają czas i ilość próbek w badaniach nad zespołem metabolicznym, zapewniając kompleksowy wgląd w powiązane schorzenia.

Multiplexing Metabolic Syndrome Biomarkers | MILLIPLEX® Assays

Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej