MABS2046

Anti-ApoB Antibody, clone 2G11

clone 2G11, from mouse

• Synonim(y): Apolipoprotein B-100
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 2G11, monoclonal
• reaktywność gatunkowa: hamster, mouse, rat
• spodziewany brak reakcji z: human
• opakowanie: antibody small pack of 25 μg
• metody: ELISA: suitable; western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NP_033823.2
• numer dostępu UniProt: E9Q414
• Warunki transportu: ambient
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: mouse ... Apob(238055)

Opis

Opis ogólny

Apolipoprotein B-100 (UniProt: E9Q414; also known as Apo B-100) is encoded by the Apob gene (Gene ID: 238055) in murine species. Apolipoprotein B is a major protein constituent of chylomicrons. Apo B particles are made up of lipids and proteins and each Apo B is bordered by a phospholipid monolayer with an inner core composed of variable amounts of triglycerides and cholesterol esters. Apo B is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved off. Two isoforms of Apo B are produced- Apo B-100 is synthesized in the liver and Apo B-48 is produced in the intestine. Apo B-100 contains the region that binds to the LDL receptor. It contains VLDL particles, VLDL remnant particles and LDL particles. On the other hand, the Apo B-48 particles are made of chylomicrons and chylomicron remnant particles. Generally, LDL particles account for approximately 90% of Apo B particles in plasma. Abnormal trapping of an Apo B is a major factor in the development of atherosclerotic lesions. When Apo B particles degrade cholesterol is retained with macrophages that induce inflammatory response. Further entry and entrapment of Apo B can promote cardiovascular pathology over time. (Ref.: Sniderman, A et al. (2010). Nat. Rev. Endocrinol. 6(6); 335-346).

Specyficzność

Clone 2G11 detects Apolipoprotein B in Mouse, Rat, and Hamster plasma and tissues. It binds to ApoB-48 and ApoB-100, but not to ApoB-39.

Immunogen

ApoB-containing lipoproteins isolated from Apoe -/- Apob 48/48 mice.

Zastosowanie

Detects Apolipoprotein B using this mouse monoclonal Anti-ApoB, clone 2G11 , Cat. No. MABS2046, is a mouse monoclonal antibody that detects Apolipoprotein B in Mouse, Rat, and Hamster and is tested for use in ELISA and Western Blotting.

Research Category
Signaling

Western Blotting Analysis: 4 µg/mL from a representative lot detected ApoB in 10 µg of rat liver and mouse liver tissue lysate.

Western Blotting Analysis: A representative lot detected ApoB in Western Blotting applications (Nguyen, A.T., et. al. (2006). Biochem Biophys Acta. 1761(2):182-5; Cheng, D., et. al. (2016). J Biol Chem. 291(45):23793-23803).

ELISA Analysis: A representative lot detected ApoB in ELISA applications (Nguyen, A.T., et. al. (2006). Biochem Biophys Acta. 1761(2):182-5).

Jakość

Evaluated by Western Blotting in rat serum.

Western Blotting Analysis: 4 µg/mL of this antibody detected ApoB in 10 µg of rat serum.

Opis wartości docelowych

~260 kDa observed; 509.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Postać fizyczna

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): does not flash
• Temperatura zapłonu (°C): does not flash