MABS1320

Anti-Serpin B13 Antibody, clone B29

clone B29, from mouse

• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: B29, monoclonal
• reaktywność gatunkowa: human, mouse
• opakowanie: antibody small pack of 25 μL
• metody: immunofluorescence: suitable; western blot: suitable
• izotyp: IgG2bκ
• numer dostępu NCBI: NP_766440
• numer dostępu UniProt: Q8CDC0
• Warunki transportu: ambient
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... SERPINB13(5275)

Opis

Opis ogólny

Serpin B13 (UniProt: Q8CDC0) is encoded by the Serpinb13 gene (Gene ID: 241196) in murine species. Serpin B13 is a cytoplasmic protein that plays a role in the proliferation or differentiation of keratinocytes. Two isoforms of Serpin B13 have been described that are generated by alternative splicing. Serpin B13 acts as a serine proteinase inhibitor and studies have shown a strong induction of serpin B13 in the pancreas of mice during the first few weeks of life. Cathepsin L and K appear to be the targets of serpin B13 action. Serpin B13 is expressed in pancreas with low levels of expression in pancreatic islets and larger expression in the exocrine pancreas. Secretion of anti-serpin B13 autoantibodies in young diabetes-prone non-obese diabetic (NOD) mice is shown to be associated with reduced inflammation in pancreatic islets and a slower progression to autoimmune diabetes. Anti-serpin B13 autoantibodies are shown to protect NOD mice against diabetes before 16 weeks of age. Reduced expression of serpin B13 autoantibodies is associated with development of type I diabetes in human before 5 years of age. Mice injected with monoclonal anti-serpin B13 display few inflammatory cells in the islets and rapid recovery from recent onset diabetes.

Specyficzność

Clone B29 detects Serpin B13 in human and murine species.

Immunogen

Purified full length mouse Serpin B13 resuspended in Complete Freund′s Adjuvant.

Zastosowanie

Anti-Serpin B13, clone B29, Cat. No. MABS1320, is a mouse monoclonal antibody that detects Serpin B13 and has been tested for use in Function Studies, Immunofluorescence, and Western Blotting.

Immunofluorescence Analysis: A representative lot detected Serpin B13 in Immunofluorescence applications (Baldzizhar, R., et. al. (2012). J Biol Chem. 288(3):1612-9).

Western Blotting Analysis: 3 ug/mL from a representative lot detected Serpin B13 in mouse serpin/HEK293 cell lysates (Courtesy of Dr. Jan Czyzyk, M.D., University of Rochester Medical Center, NY).

Western Blotting Analysis: A representative lot detected Serpin B13 in Western Blotting applications (Baldzizhar, R., et. al. (2012). J Biol Chem. 288(3):1612-9; Czyzyk, J., et. al. (2012). J Immunol. 188(12):6319-27).

Affects Function Analysis: A representative lot detected Serpin B13 in Affects Function applications (Baldzizhar, R., et. al. (2012). J Biol Chem. 288(3):1612-9).

Research Category
Signaling

Jakość

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .

Opis wartości docelowych

44.32 kda calculated.

Postać fizyczna

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Ta strona może zawierać tekst przetłumaczony maszynowo.