MABS1132

Anti-CDKL5 Antibody, clone 8F3.1

clone 8F3.1, from mouse

• Synonim(y): Cyclin-dependent kinase-like 5, Serine/threonine-protein kinase 9
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: 8F3.1, monoclonal
• reaktywność gatunkowa: mouse, rat
• reaktywność gatunkowa (przewidywana na podstawie homologii): human (based on 100% sequence homology)
• metody: western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NP_001032420
• numer dostępu UniProt: O76039
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: mouse ... Cdkl5(382253)

Opis

Opis ogólny

Cyclin-dependent kinase-like 5 (EC 2.7.11.22; UniProt O76039; also known as Serine/threonine-protein kinase 9) is encoded by the CDKL5 (also known as EIEE2, STK9) gene (Gene ID 6792) in human. CDKL5/STK9 is a serine/threonine protein kinase widely distributed in all tissues, with highest levels in thymus, testes, and brain, where it is found localized in the nucleus and dendrite of neurons. CDKL5/STK9 expression is found peaked in the early postnatal period and Cdkl5-knockdown in mice leads to autistic-like behavior, dendritic hypotrophy and impairment of neural circuit communication. Likewise, human CDKL5 gene mutations are linked to X-linked neurological developmental disorders involving severe mental retardation and seizures appearing in the first postnatal months. Point mutations in CDKL5 have also been reported in patients with atypical variants of Rett syndrome (RTT), infantile spasms and severe neurodevelopmental retardation.

Specyficzność

Expected to react with both spliced isoforms of human CDKL5 reported by UniProt (O76039).

Immunogen

Epitope: Internal.

GST-tagged recombinant human CDKL5 internal fragment.

Zastosowanie

Anti-CDKL5 Antibody, clone 8F3.1 is an antibody against CDKL5 for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected CDKL5 in 10 µg of rat hippocampus tissue lysate.

Jakość

Evaluated by Western Blotting in mouse cerebellum tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CDKL5 in 10 µg of mouse cerebellum tissue lysate.

Opis wartości docelowych

~115 kDa observed. 115.5 kDa (isoform 1) and 107.5 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

Postać fizyczna

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable