MABN2440

Anti-ABCA4 Antibody, clone 5B4

clone 5B4, from mouse

• Synonim(y): Retinal-specific ATP-binding cassette transporter, ATP-binding cassette sub-family A member 4, RIM ABC transporter, RIM protein, RmP, Stargardt disease protein
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: 5B4, monoclonal
• reaktywność gatunkowa: bovine, human
• reaktywność gatunkowa (przewidywana na podstawie homologii): mouse (based on 100% sequence homology)
• opakowanie: antibody small pack of 25 μg
• metody: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• izotyp: IgG2bκ
• numer dostępu NCBI: NP_000341.2
• numer dostępu UniProt: P78363
• Warunki transportu: ambient
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... ABCA4(24)

Opis

Opis ogólny

Retinal-specific ATP-binding cassette transporter (UniProt: P78363; also known as ATP-binding cassette sub-family A member 4, ABCA4, RIM ABC transporter, RIM protein, RmP, Stargardt disease protein) is encoded by the ABCA4 (also known as ABCR) gene (Gene ID: 24) in human. ABCA4 is a multi-pass membrane protein that is exclusively expressed in retina and is found in the rims of rod photoreceptor cells. It contains two ABC transporter domains (aa 929-1160 and 1938-2170). In the visual cycle, it act as a retinal extruder or retinal-phosphatidylethanolamine flippase to facilitate the removal of all-trans-retinal from disc membranes following the photobleaching of rhodopsin. Mutations in ABCA4 gene are reported to cause Stargardt disease, which is characterized by decreased central vision, atrophy of the macula and underlying retinal pigment epithelium, and frequent presence of prominent flecks in the posterior pole of the retina. It has also been linked to age-related macular degeneration and retinitis pigmentosa.

Specyficzność

Clone 5B4 detects Retinal-specific ATP-binding cassette transporter protein in human and bovine. It targets an epitope within 249 amino acids from the N-terminal half.

Immunogen

GST-tagged recombinant fragment corresponding to 249 amino acids from the N-terminal half of human Retinal-specific ATP-binding cassette transporter.

Zastosowanie

Anti-ABCA4, clone 5B4, Cat. No. MABN2440, is a highly specific mouse monoclonal antibody that targets Retinal-specific ATP-binding cassette transporter and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Immunocytochemistry Analysis: A representative lot detected ABCA4 in Immunocytochemistry applications (Ahn, J., et. al. (2003). J Biol Chem. 278(41):39600-8).

Western Blotting Analysis: A representative lot detected ABCA4 in Western Blotting applications (Ahn, J., et. al. (2003). J Biol Chem. 278(41):39600-8; Bungert, S., et. al. (2001). J Biol Chem. 276(26):23539-46).

Immunoprecipitation Analysis: A representative lot detected ABCA4 in Immunoprecipitation applications (Ahn, J., et. al. (2003). J Biol Chem. 278(41):39600-8; Bungert, S., et. al. (2001). J Biol Chem. 276(26):23539-46).

Research Category
Neuroscience

Jakość

Evaluated by Western Blotting in human retina tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected ABCA4 in 10 µg of human retina tissue lysate.

Opis wartości docelowych

250 kDa observed; 255.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Postać fizyczna

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): does not flash
• Temperatura zapłonu (°C): does not flash